Name: Recombinant Porphyromonas gingivalis Gingipain R1 (rgpA), partial
Brand: CUSABIO
SKU: CSB-EP338957PQP

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

rgpA

Uniprot No.

P28784

Research Area

others

Alternative Names

rgpA; rgp1Gingipain R1; EC 3.4.22.37; Arg-gingipain; Gingipain 1; RGP-1

Species

Porphyromonas gingivalis

Source

E.coli

Expression Region

228-720aa

Target Protein Sequence

YTPVEEKQNGRMIVIVAKKYEGDIKDFVDWKNQRGLRTEVKVAEDIASPVTANAIQQFVKQEYEKEGNDLTYVLLVGDHKDIPAKITPGIKSDQVYGQIVGNDHYNEVFIGRFSCESKEDLKTQIDRTIHYERNITTEDKWLGQALCIASAEGGPSADNGESDIQHENVIANLLTQYGYTKIIKCYDPGVTPKNIIDAFNGGISLVNYTGHGSETAWGTSHFGTTHVKQLTNSNQLPFIFDVACVNGDFLFSMPCFAEALMRAQKDGKPTGTVAIIASTINQSWASPMRGQDEMNEILCEKHPNNIKRTFGGVTMNGMFAMVEKYKKDGEKMLDTWTVFGDPSLLVRTLVPTKMQVTAPAQINLTDASVNVSCDYNGAIATISANGKMFGSAVVENGTATINLTGLTNESTLTLTVVGYNKETVIKTINTNGEPNPYQPVSNLTATTQGQKVTLKWDAPSTKTNATTNTARSVDGIRELVLLSVSDAPELLRS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

68.0kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-B2M-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Porphyromonas gingivalis rgpA protein is encoded by a recombinant DNA that was cloned into the expression vector and then transformed into the E.coli that supports the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 6xHis-B2M tag gene to the gene fragment coding for the 228-720aa of the Porphyromonas gingivalis rgpA protein. After purification, the product is the recombinant Porphyromonas gingivalis rgpA protein. This recombinant rgpA protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This rgpA protein ran to the molecular weight of about 70 kDa under SDS-PAGE condition.

Porphyromonas gingivalis is a well-known bacterium that causes periodontitis and gingivitis. Inflammagens of porphyromonas gingivalis are associated with the development of various inflammatory conditions. Gingipains consist of rgpA and rgpB, and Lys-gingipain (Kgp). They play a central role in the virulence of this organism. Gingipain R1 is synergistically with RgpB to catalyze the maturation of fimbrial subunits, such as FimA. In recent years, findings suggested that Gingipain R1 (rgpA) from porphyromonas gingivalis has major effects on blood clot morphology and mechanics.

Customer Reviews and Q&A

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■ Q&A

Can you please verify that the bioactivity has not been tested. I would like to remove the 6His+B2M, N-terminus tag. Do you have a recommended procedure for the removal?

Very nice to receive your inquiry. The product details are confirmed as below.
1. General Information
Code: CSB-EP338957PQP
Name: Recombinant Porphyromonas gingivalis Gingipain R1(rgpA),partial
Expression Region: 228-720aa; Partial
Tag Info: N-terminal 6xHis-B2M-tagged
Expression Sequence:

Delivery Form: Liquid
Storage Buffer: 0.2 μm sterile filtered 10 mM Tris-HCl,1 mM EDTA, pH 8.0, 50% glycerole
Endotoxin Level: <1.0 EU per 1μg of the protein by the LAL method
Aseptic Processing: Yes
2. Tag Removal
There is no restriction site on this N-terminal 6xHis-B2M-tagged protein so the tag can't be removed. Generally the tag won't make big influence on the protein.
If you are concerned and would like to remove the tag, we need to re-design primer and re-express this protein.
3. Activity
We have't tested the activity of this protein so we can't 100% guarantee that is has activity. The protein we provide is fragment which is purified under mild conditions.
If the site that you need is contained in this region (228-720aa), then this protein should be active in theory.

Please provide the following information for CSB-EP338957PQP:
Have you performed tag removal for this protein before? We want to remove the tag.
Do you have the full-length protein? If yes, can you please provide sequence, pricing, etc. We're looking for this protein but with no tag. Do you have other options (species or expression regions) that you have successfully expressed before with no tag (removed tag)? If yes, please provide the catalog, pricing, sequence, etc.

Thanks for your inquiry.
Recombinant Porphyromonas gingivalis Gingipain R1(rgpA),partial
CSB-EP338957PQP >> E.coli
Sorry, we haven't tried to remove the tag of this protein before, But for some applications, the small tag such as His-Tag may have very less impact on protein activity or function, so probably there is no need to remove them. Therefore, you can consider to order YP protein. we will send you the COA of the previous Lot for reference.
In addition, is it convenient to disclose which kind of application will you use this protein for ? If it must be no-tag, we can also try to remove the tag, please remark your requirement when placing the order.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.
If we are failed in removing the tag, we will provide the fusion protein and won't charge any extra fees for tag removal.
② As you also asked about the full length protein, we can provide risk-free custom service for the full length protein, please check the details as follows:
Recombinant Porphyromonas gingivalis Gingipain R1(rgpA)
CSB-YP338957PQP(A4) >> Yeast
CSB-EP338957PQP(A4) >> E.coli
CSB-BP338957PQP(A4) >> Baculovirus
CSB-MP338957PQP(A4) >> Mammalian cell
Expression Region: 228-991aa; Mature full length protein.
Tag information: Tag type will be determined during the manufacturing process.
We can try to express your desired tag first if you have special request for the tag, but as we can't guarantee, if it's failed, we can provide protein with other tag.
Sequence: The same as CSB-EP338957PQP
Reference:http://www.uniprot.org/uniprot/P28784
If we can successfully express this protein, you could pay the corresponding fees and we will provide the purified protein.
If we are failed in expression this protein, you don't need to pay any fees. However you could pay for the gene synthesis and subcloning to purchase the constructed plasmid and strains if you need.
PS: The expression plasmid is not for sale if this project is successful.
If you need the untagged protein, there will be extra charges for tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.
If we are failed in removing the tag, we will provide the fusion protein and won't charge any extra fees for tag removal.

Target Background

Function

Thiol protease. Acts synergistically with RgpB to catalyze the maturation of fimbrial subunits, such as FimA. Its proteolytic activity is a major factor in both periodontal tissue destruction and in evasion of host defense mechanisms (Probable).

Subcellular Location

Secreted.

Protein Families

Peptidase C25 family

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