Recombinant Pseudomonas aeruginosa UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)

Thanks for your inquiry.
Recombinant Pseudomonas aeruginosa UDP-3-O-acyl-N-acetylglucosamine deacetylase(lpxC)
CSB-BP409457PZL >> Baculovirus
CSB-MP409457PZL >> Mammalian cell
Expression Region: 1-303aa; Full length.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MIKQRTLKNIIRATGVGLHSGEKVYLTLKPAPVDTGIVFCRTDLDPVVEIPARAENVGETTMSTTLVKGDVKVDTVEHLLSAMAGLGIDNAYVELSASEVPIMDGSAGPFVFLIQSAGLQEQEAAKKFIRIKREVSVEEGDKRAVFVPFDGFKVSFEIDFDHPVFRGRTQQASVDFSSTSFVKEVSRARTFGFMRDIEYLRSQNLALGGSVENAIVVDENRVLNEDGLRYEDEFVKHKILDAIGDLYLLGNSLIGEFRGFKSGHALNNQLLRSLIADRDAWEVVTFEDARTAPISYMRPAAAV

Sorry, we haven't tried to remove the tag of these proteins. Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order.
We can try enzyme digestion, the C-terminal tag can guaranteed to be removed successfully, but we can't guarantee N-terminal tag removal100% successfully. The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.