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Recombinant Pseudomonas putida Phthalate 4,5-dioxygenase oxygenase subunit (pht3)

Recombinant Pseudomonas putida Phthalate 4,5-dioxygenase oxygenase subunit (pht3)

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Code	CSB-EP313137FFZ
Abbreviation	Recombinant Pseudomonas putida pht3 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

pht3

Uniprot No.

Q05183

Research Area

others

Species

Pseudomonas putida (Arthrobacter siderocapsulatus)

Source

E.coli

Expression Region

1-439aa

Target Protein Sequence

MLTPEENLLLCRVEGDAPMGQMMRRHWTPVCLLEEVSEPDGTPVRARLFGEDLVVFRDTDGRVGVMDEYCPHRRVSLIYGRNENSGLRCLYHGWKMDVDGNVVEMVSEPAASNMCQKVKHTAYKTREWGGFVWAYMGPQDAIPEFVPPAWAPHEHVRVSIAKAIIPCNWAQILEGAIDSAHSSSLHSSDFVPARVGGAEATSKNWLRPSTDKAPRMQVERTSYGFRYAALRRPIQNAATSEYVRSTVFVAPATALIPPNNLYNVANINVPIDDTHTAFYFMAWGNPDNTPETETWRKFLGQQVGIDLDDSYRPLRNDGNRFFQDREAMKNGNFTGIKGFPNQDIAMWVTMGPIADRSDERLGASDLAVVEFRRVMLDALAAFQAGESAIGTGEKAIPSRICSFQAIVSKDIDWRDYQARYVWALDDANIVAEPDYEVHT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

56.7 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To achieve the expression of the recombinant Pseudomonas putida (Arthrobacter siderocapsulatus) pht3 protein in e.coli cells, a DNA fragment encoding the Pseudomonas putida (Arthrobacter siderocapsulatus) pht3 protein (1-439aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the pht3 protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Pseudomonas putida (Arthrobacter siderocapsulatus) pht3 protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Pseudomonas putida (Arthrobacter siderocapsulatus) pht3 protein is greater than 85%, measured by SDS-PAGE.