Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE)

Code CSB-YP838038CMB
Size $436
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
nanE
Uniprot No.
Research Area
Others
Species
Clostridium perfringens (strain 13 / Type A)
Source
Yeast
Expression Region
1-221aa
Target Protein Sequence
MLDVVKGNLIVSCQALSDEPLHSSFIMGRMAIAAKQGGAAAIRAQGVNDINEIKEVTKLPIIGIIKRNYDDSEIYITPTMKEVDELLKTDCEMIALDATKRKRPNGENVKDLVDAIHAKGRLAMADISTLEEGIEAEKLGFDCVSTTLSGYTPYSKQSNSVDFELLEELVKTVKIPVICEGRINTPEELKKALDLGAYSAVVGGAITRPQQITKRFTDILK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
25.7 kDa
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) is produced using a yeast expression system. The expression construct encodes the full-length nanE protein from Clostridium perfringens, spanning amino acids 1 to 221, and includes a C-terminal 6xHis-tag for purification purposes. The production process involves introducing the expression construct into yeast cells and allowing them to express and synthesize the recombinant nanE protein. The purified recombinant nanE protein exhibits a purity of up to 85%, as determined by SDS-PAGE analysis. On the gel, the nanE protein migrates as a band with an approximate molecular weight of 28 kDa.

nanE mainly catalyzes the conversion of N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P), playing crucial in the biosynthesis of N-acetylneuraminic acid (Neu5Ac), which is an essential component of various cellular structures such as glycoproteins, glycolipids, and polysaccharides.

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Target Background

Function
Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P).
Protein Families
NanE family
Database Links

KEGG: cpe:CPE0184

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