Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

HINT1

Uniprot No.

P80912

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

HINT1; HINTHistidine triad nucleotide-binding protein 1; EC 3.-.-.-; Adenosine 5'-monophosphoramidase; P13.7

Species

Oryctolagus cuniculus (Rabbit)

Source

Mammalian cell

Expression Region

2-126aa

Target Protein Sequence

ADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDQCLAFHDISPQAPTHFLVIPKKHISQISAAEDADESLLGHLMIVGKKCAADLGLKKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMNWPPG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

17.6kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Rabbit HINT1 contains amino acids 2-126. The expected molecular weight for the HINT1 protein is calculated to be 17.6 kDa. This HINT1 protein is produced using mammalian cell expression system. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of HINT1, making it easier to detect and purify the HINT1 recombinant protein in the later stages of expression and purification.

The rabbit histidine triad nucleotide-binding protein 1 (HINT1) is a vital player in nucleotide metabolism, contributing to purine nucleotide recycling within cells. Its main functions extend to regulating cellular processes such as signal transduction, apoptosis, and genomic stability. Research on rabbit HINT1 focuses on unraveling its precise roles in nucleotide metabolism and cellular signaling pathways. Additionally, investigations explore the potential implications of HINT1 in various physiological and pathological conditions. Understanding the functions of rabbit HINT1 provides valuable insights into conserved biological processes across species, shedding light on its significance in maintaining cellular homeostasis and its potential as a target for therapeutic interventions.

Target Background

Function

Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase. Hydrolyzes Met-AMP, His-AMP, Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RANGAP1 and RGS17.

Gene References into Functions

structure of the rabbit HINT1-adenosine complex is reported at 1.10 A resolution, which is one of the highest resolutions obtained for a HINT1 structure PMID: 21697598

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

HINT family

Tissue Specificity

Widely expressed.

Database Links

KEGG: ocu:100009302

STRING: 9986.ENSOCUP00000005709

UniGene: Ocu.3078

Code	CSB-MP301425RB
Abbreviation	Recombinant Rabbit HINT1 protein
MSDS	MSDS Datasheet
Size	$590
	Order now
Image	Warning: preg_replace(): Unknown modifier 'a' in /www/wwwroot/cusabio.com/caches/caches_template/20141113/content/show_product_protein.php on line 156
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Rabbit Histidine triad nucleotide-binding protein 1 (HINT1)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage