Recombinant Rat Adenosine kinase (Adk)

Code CSB-YP734122RA
MSDS
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Source Yeast
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Code CSB-EP734122RA
MSDS
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Source E.coli
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Code CSB-EP734122RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP734122RA
MSDS
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Source Baculovirus
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Code CSB-MP734122RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Adk
Uniprot No.
Alternative Names
AdkAdenosine kinase; AK; EC 2.7.1.20; Adenosine 5'-phosphotransferase
Species
Rattus norvegicus (Rat)
Expression Region
1-361
Target Protein Sequence
MAAADEPKPK KLKVEAPEAL SENVLFGMGN PLLDISAVVD KDFLDKYSLK PNDQILAEDK HKELFDELVK KFKVEYHAGG STQNSMKVAQ WMIQEPHRAA TFFGCIGIDK FGEILKSKAA DAHVDAHYYE QNEQPTGTCA ACITGGNRSL VANLAAANCY KKEKHLDLEN NWMLVEKARV YYIAGFFLTV SPESVLKVAR YAAENNRTFT LNLSAPFISQ FFKEALMEVM PYVDILFGNE TEAATFAREQ GFETKDIKEI ARKTQALPKV NSKRQRTVIF TQGRDDTIVA TGNDVTAFPV LDQNQEEIVD TNGAGDAFVG GFLSQLVSNK PLTECIRAGH YAASVIIRRT GCTFPEKPDF H
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Gene References into Functions
  1. ADK was associated with astrogliosis after TBI, its inhibition in reactive astrocytes ameliorated astrogliosis-induced neuronal death. Our findings extended the current knowledge on the role of ADK in astrogliosis, and also provided new evidence for the TBI treatment. PMID: 26983602
  2. Adenosine kinase inhibition selectively promotes rodent and porcine islet beta-cell replication PMID: 22345561
  3. Adenosine kinase is an important mediator of adenosine attenuation of cardiomyocyte hypertrophy. PMID: 21335462
  4. In the developing cochlea, ADK is strongly expressed in multiple cell types at birth and reaches its peak level of expression at postnatal day 21; it is involved in purine salvage pathways for nucleotide synthesis in the adult cochlea. PMID: 20648650
  5. Expression level of adenosine kinase in rat tissues. Lack of phosphate effect on the enzyme activity. PMID: 11833783
  6. Data show that insulin stimulates AK gene expression through a series of events occurring sequentially; this includes activation of the MAPK cascade and subsequent phosphorylation of Elk-1 followed by increased expression of c-fos and c-jun genes PMID: 12729803
  7. biochemical analysis of adenosine kinase from rat liver PMID: 17065073
  8. The reaction catalyzed by adenosine kinase (adenosine + ATP --> AMP + ADP) can be easily reversed when coupled with adenosine deaminase, which transforms adenosine into inosine and ammonia. PMID: 18600555

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Subcellular Location
Nucleus.
Protein Families
Carbohydrate kinase PfkB family
Database Links
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