Recombinant Rat Beta-hexosaminidase subunit beta (Hexb)

Code CSB-YP738123RA
MSDS
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Source Yeast
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Code CSB-EP738123RA
MSDS
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Source E.coli
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Code CSB-EP738123RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP738123RA
MSDS
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Source Baculovirus
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Code CSB-MP738123RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hexb
Uniprot No.
Alternative Names
Hexb; Beta-hexosaminidase subunit beta; EC 3.2.1.52; Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta
Species
Rattus norvegicus (Rat)
Expression Region
24-537
Target Protein Sequence
LVAPFGL QPALWPMPRS VQVFPRLLYI SPENFQIDNS PNSTAGPSCS LLLEAFRRYY NYIFGFYKRH HGPAKFQDKP QLEKLLVFIN LEPQCDAFPS MSSDESYSLL VQEPVALLKA NEVWGALRGL ETFSQLVYQD AYGTFTINES TIADSPRFPH RGILIDTSRH YLPVKTIFKT LDAMAFNKFN VLHWHIVDDQ SFPYQSITFP ELSNKGSYSL SHVYTPNDIH MVLEYARLRG IRVIPEFDSP GHTQSWGKGQ KNLLTPCFIQ KIRTQKVGPV DPSLNTTYVF FDTFFKEISR VFPDQFIHLG GDEVEFECWA SNPNIQNFMK KKGFGNNFRR LESFYIKKIL DIITSLKKSS IVWQDVFDDQ VELQPGTVVE VWKSENYLNE LAQVTASGFP AILSAPWYLD LISYGQDWRN YYKAEPLNFE GSEKQKQLVI GGEACLWGEY VDATNLIPRL WPRASAVGER LWSPRIITNL ENAYRRLAVH RCRMVSRGIA AQPLFTGYCN YENKMEK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
Subcellular Location
Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
Protein Families
Glycosyl hydrolase 20 family
Database Links

KEGG: rno:294673

UniGene: Rn.203067

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