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Recombinant Rat Calcium calmodulin-dependent 3,5 cyclic nucleotide phosphodiesterase 1C (Pde1c)

Recombinant Rat Calcium calmodulin-dependent 3,5 cyclic nucleotide phosphodiesterase 1C (Pde1c)

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Citations (1)

Code	CSB-EP720512RA
Abbreviation	Recombinant Rat Pde1c protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Pde1c

Uniprot No.

Q63421

Research Area

Signal Transduction

Alternative Names

Cam-PDE 1C;3',5'-cyclic-AMP phosphodiesterase (EC:3.1.4.-By similarity);3',5'-cyclic-GMP phosphodiesterase

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

1-768aa

Target Protein Sequence

MTDTSHKKEGFKKCRSATFSIDGYSFTIVANEAGDKNARPLARFSRSKSQNCLWNSLIDGLTGNVKEKPRPTIVQDTRPPEEILADELPQLDSPEALVKTSFRLRSLVKQLERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMVGLSYPPAVIDALKDVDTWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHHLSAAYRLLQEDEEMNILVNLSKDDWREFRTLVIEMVMATDMSCHFQQIKAMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSINSSDAKRSGVKSSGSEGSAPINNSVIPVDYKSFKATWTEVVQINRERWRAKVPKEEKAKKEAEEKARLAAEEKQKEMEAKSQAEQGTTSKAEKKTSGETKGQVNGTRTSKGDNPRGKNSKGDKAGEKQQNGDLKDGKNKADKKDHSNTGNESKKADGTKKRSHGSPAPSTSSTSRLTLPVIKPPLRHFKRPAYASSSYAPSVPKKTDDHPVRYKMLDQRIKIKKIQNISHHWNKK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

92.6 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Enrich your research in signal transduction with our high-quality Recombinant Rat Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C (Cam-PDE 1C). This product is derived from the 'Pde1c' gene of Rat species, functioning as a calcium-calmodulin-dependent enzyme involved in the regulation of cyclic nucleotide content and thus, affecting the cellular responses triggered by these signaling nucleotides.

Generated from E.coli, the protein replicates the full length, capturing all 768 amino acids of the native protein. To enhance its functionality, our recombinant protein is tagged with a N-terminal 6xHis tag for simplified purification and detection. Achieving a purity greater than 85% as validated by SDS-PAGE, our recombinant protein ensures reliable results. We offer the Recombinant Rat Cam-PDE 1C protein in two different forms - liquid or lyophilized powder, providing flexibility for your experimental setup.

Citations

Regulation of cerebral blood flow boosts precise brain targeting of vinpocetine-derived ionizable-lipidoid nanoparticles X Bian, L Yang, D Jiang, AJ Grippin, Y Ma, S Wu, L Wu, X Wang, Z Tang, K Tang, W Pan,Nature communications,2024

Customer Reviews and Q&A

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Target Background

Function

Calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP. Modulates the amplitude and duration of the cAMP signal in sensory cilia in response to odorant stimulation, hence contributing to the generation of action potentials. Regulates smooth muscle cell proliferation. Regulates the stability of growth factor receptors, including PDGFRB.

Gene References into Functions

In cultured rat aortic SMCs, PDE1C deficiency or PDE1 inhibition attenuated SMC proliferation and migration. PDE1C plays a critical role in regulating the stability of growth factor receptors, endocytosis and lysosome-dependent degradation. PMID: 25608528
Suggest that PDE1C plays a critical role in regulating collagen homeostasis during pathological vascular remodeling. PMID: 21148428

Protein Families

Cyclic nucleotide phosphodiesterase family, PDE1 subfamily

Tissue Specificity

Highly expressed in olfactory epithelium and at moderate levels, in cerebellum, as well as weakly in forebrain, testis, heart and lung. In the olfactory epithelium, expressed by sensory neurons, but not epithelial cells.

Database Links

KEGG: rno:81742

STRING: 10116.ENSRNOP00000017531

UniGene: Rn.10289

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