Recombinant Rat Cyclic AMP-dependent transcription factor ATF-2 (Atf2)

Code CSB-YP002270RA
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Source Yeast
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Code CSB-EP002270RA
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Source E.coli
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Code CSB-EP002270RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002270RA
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Source Baculovirus
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Code CSB-MP002270RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Atf2
Uniprot No.
Alternative Names
Atf2Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; EC 2.3.1.48; Activating transcription factor 2; cAMP response element-binding protein CRE-BP1
Species
Rattus norvegicus (Rat)
Expression Region
1-487
Target Protein Sequence
MSDDKPFLCT APGCGQRFTN EDHLAVHKHK HEMTLKFGPA RNDSVIVADQ TPTPTRFLKN CEEVGLFNEL ASPFENEFKK ASEDDIKKMP LDLSPLATPI IRSKIEEPSV VETTHQDSPL PHPESTTNDE KEIPLAQTAQ PTSAIVRPAS LQVPNVLLTS SDSSVIIQQA VPSPTSSTVI TQAPSSNRPI VPVPGPFPLL LHLPNGQTMP VAIPASITSS NVHVPAAVPL VRPVTMVPSV PGIPGPSSPQ PVQSEAKMRL KAALTQQHPP VTNGDTVKGH GSGLVRAQSE ESRPQSLQQP ATSTTETPAS PAHTTPQTQN TSGRRRRAAN EDPDEKRRKF LERNRAAASR CRQKRKVWVQ SLEKKAEDLS SLNGQLQSEV TLLRNEVAQL KQLLLAHKDC PVTAMQKKSG YHTADKDDSS EDLSVPSSPH TEAIQHSSVS TSNGVSSTSK TEAGATSVLT QMADQSTEPA LSQIVMAPSS QAQPSGS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
Gene References into Functions
  1. This study demonstrated that ATF2 participates in nerve injury-induced tactile allodynia and thermal hyperalgesia in rats. PMID: 29921170
  2. The results suggest that the increase in uncoupling protein 1 (UCP1) content may occur via p38 MAPK and ATF2. PMID: 26781764
  3. Following unilateral intracochlear stimulation, study quantified an increase of Fos expression with a simultaneous and locally corresponding decrease of pATF2 expression in major auditory brainstem regions of both hearing and deaf rats PMID: 26601778
  4. Caspase-3 is a target gene of c-Jun:ATF2 heterodimers during apoptosis induced by activity deprivation in cerebellar granule neurons. PMID: 21996423
  5. phosphorylation of ATF-2 by c-Jun N-terminal kinase is associated with retinal ganglion cell death after axon damage. PMID: 21300140
  6. JNK/ATF2 pathway is involved in iodinated contrast media-induced apoptosis PMID: 19923798
  7. These results indicate that induced ATF-2/JunD association following polyamine depletion represses CDK4 transcription, thus contributing to the inhibition of intestinal epithelial cell growth. PMID: 20181929
  8. role in regulating tyrosine hydroxylase PMID: 12196528
  9. ATF-2 is deeply involved in amelogenesis and is related to the proliferation, differentiation, secretion and transition zones. PMID: 15218628
  10. ATF-2 activation plays a role in TGF-beta1-induced cardiac hypertrophic responses in cardiac myocytes culture system. PMID: 16125722
  11. polyamines modulate the stability of ATF-2 mRNA by altering cytoplasmic HuR levels and that polyamine-modulated ATF-2 expression plays a critical role in regulating epithelial apoptosis PMID: 17804813
  12. ATF2 overexpression in nucleus accumbens produces increases in emotional reactivity. PMID: 18305237
  13. Each member of AP-1 is expressed differently and that ATF-2 is more active than c-Jun and Fra-2 in physiological conditions in healthy rat jejunum. PMID: 18320311
  14. Results suggest that potassium deprivation-induced neuronal apoptosis is mediated by concurrent upregulation of c-Jun/ATF2 heterodimerization and downregulation of c-Fos expression. PMID: 19255142

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Subcellular Location
Nucleus. Cytoplasm. Mitochondrion outer membrane.
Protein Families
BZIP family, ATF subfamily
Database Links
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