Recombinant Rat Diacylglycerol kinase alpha(Dgka)

Code CSB-YP006832RA
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Source Yeast
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Code CSB-EP006832RA
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Source E.coli
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Code CSB-EP006832RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006832RA
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Source Baculovirus
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Code CSB-MP006832RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Dgka
Uniprot No. P51556
Alternative Names Dgka; Dagk; Dagk1; Diacylglycerol kinase alpha; DAG kinase alpha; EC 2.7.1.107; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha
Species Rattus norvegicus (Rat)
Expression Region 1-727
Target Protein Sequence MAKDKGLISP EDFAQLQKYI DYSTKSVSDV LKVFEMNKYC QGDEIGYLGF EQFLKMYLEV EEVPHHLCWT LFWSFHSSQD LDEETESKAN VICLSDVYCY FTLLEGGSPE DKLEFTFKLY DMDRNGILDS TEVEKIILQM MRVAEYLDWD VSELRPILQE MMKEMDRDGS GCVSLAEWVR AGATTVPLLV LLGIDMTMKD DGHHIWRPKR FSRPVYCNLC ELSIGLGKQG LSCNLCKYIV HDHCAMKAQP CEVSTYAKSR KDIGVQPHVW VRGGCHSGRC DRCQKKIRTY HSLTGLHCVW CHLEIHDDCL QAVGPECDCG LLRDHILPPC SIYPRVLVSG QECKQKTTDV TSLCTPEAFR IEPVSNTHPL LVFINPKSGG KQGQSVLWKF QYILNPRQVF NLKDGPEPGL RFFKDVPQFR VLVCGGDGTV GWILETIDKA NFPIVPPVAV LPLGTGNDLA RCLRWGRGYE GENLRKILKD IEISKVVYLD RWLLEVIPQQ NGEKSDPVPS QIINNYFSIG VDASIAHRFH LMREKYPEKF NSRMKNKLWY LEFATSESIF STCKKLEESV TVEICGKLLD LSDLSLEGIA VLNIPSMHGG SNLWGDTKRP HGDTCGINQA LGSVAKIITD PDILKTCVPD MSDKRLEVVG IEGVIEMGQI YTRLKSAGHR LAKCSEITFQ TTKTLPMQVD GEPWMQAPCT IKITHKNQMP MLMGPAPSSS NFFGFWS
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.
Gene References into Functions
  1. The DGKalpha localize predominantly to the nucleus of ganglion cells. PMID: 23467923
Subcellular Location Cytoplasm, cytosol.
Protein Families Eukaryotic diacylglycerol kinase family
Tissue Specificity Lymphocytes and oligodendroglial cells.
Database Links

KEGG: rno:140866

STRING: 10116.ENSRNOP00000038628

UniGene: Rn.1840

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