Recombinant Rat F-box only protein 32 (Fbxo32)

Code CSB-YP852812RA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP852812RA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP852812RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP852812RA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP852812RA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fbxo32
Uniprot No.
Alternative Names
Fbxo32F-box only protein 32; Atrogin-1; Muscle atrophy F-box protein; MAFbx
Species
Rattus norvegicus (Rat)
Expression Region
1-350
Target Protein Sequence
MPFLGQDWRS PGQSWVKTAD GWKRFLDEKS GTFVSDLSSY CNKENLFNSL NYDVAAKKRK KDIQNSKTKT QYFHQEKWIY VHKGSTKERH GYCTLGEAFN RLDFSTAILD SRRFNYVVRL LELIAKSQLT SLSGIAQKNF MNILEKVVLK VLEDQQNIRL IRELLQTLYT SLCTLVQRVG KSVLVGNINM WVYRMETTLH WQQQLNSIQI SRPAFKGLTI TDLPVCLQLN IMQRLSDGRD LVSLGQAAPD LHVLSEDRLL WKRLCQYHFS ERQIRKRLIL SDKGQLDWKK MYFKLVRCYP RREQYGVTLQ LCKHCHILSW KGTDHPCTAN NPESCSVSLS PQDFINLFKF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Gene References into Functions
  1. AMPK plays an important role in the inhibition of cardiomyocyte hypertrophy by activating protein degradation via FOXO3a/MAFbx signaling pathway. PMID: 27521792
  2. Suggest role for atrogin-1 up-regulation in simvastatin-induced heart mitochondria dysfunction. PMID: 25300705
  3. miR-19a/b family regulates phenotypes of cardiomyocytes via suppression of multiple direct target genes such as atrogin1. PMID: 24117217
  4. Co-expression of myocardin with atrogin-1 inhibited atrogin-1-induced myogenin expression. PMID: 23526547
  5. Overexpression of atrogin-1 results in marked alterations of gene expression profiles that are associated with cardiomyocyte survival, proliferation, inflammation, metabolism and hypertrophy. PMID: 23335977
  6. Resveratrol significantly inhibited PDGF-stimulated proliferation and cellular hypertrophy, which was also associated with improvements in atrogin-1 levels. PMID: 22146233
  7. Stability of F-box protein atrogin-1 is regulated by p38 mitogen-activated protein kinase pathway in cardiac H9c2 cells. PMID: 21691063
  8. In primary cultured rat cardiomyocytes, phenylephrine-induced activation of nuclear factor-kappaB and hypertrophy were significantly suppressed by MAFbx knockdown but were partially rescued by overexpression of nuclear factor-kappaB p65. PMID: 21617130
  9. FOXO3a mediates a reciprocal communication between the IRS-1/PI3K/Akt and IRS-2/MEK/ERK pathways that coordinates AT-1 and ubiquitin expression during muscle atrophy. PMID: 20371624
  10. Data suggest that the inhibitory effect of chronic arthritis on skeletal muscle is higher in fast glycolytic than in slow oxidative muscle and that IGF-I administration attenuates this effect and decreases atrogin-1 and IGFBP-3 gene expression. PMID: 20519361
  11. The high expression of MAFbx mRNA and protein, especially their persistent increases from 4 to 15 weeks after nerve reinnervation, is related to muscle atrophy and the decrease of muscle function. PMID: 19728616
  12. IGF-I inhibits atrogin-1/MAFbx expression PMID: 15284206
  13. Atrogin-1 is involved in the early stages of skeletal muscle regeneration in rats, independently of calcineurin. PMID: 15726428
  14. FBXO25 is a novel F-box protein analogous to atrogin-1, which is not involved in muscle atrophy. PMID: 16714087
  15. ES based on chronaxie values and applied to denervated muscles using surface electrodes was able to reduce atrogin-1 gene expression, which is related to muscular atrophy. PMID: 17034040
  16. MAFbx in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading is described. PMID: 17622304
  17. Alcohol increased markers of oxidative stress, including atrogin-1, by approximately 60-fold, which was attenuated by procysteine supplementation. PMID: 17721978
  18. Alternate name for this locus: MAFbx. Alias: MAFbx. PMID: 11679633

Show More

Hide All

Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Specifically expressed in cardiac and skeletal muscle.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1