Recombinant Rat Neural cell adhesion molecule 1 (Ncam1), partial

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Code CSB-EP015511RA
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Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Neuroscience
Alternative Names
Ncam1; Ncam; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD antigen CD56
Species
Rattus norvegicus (Rat)
Source
E.coli
Expression Region
20-721aa
Target Protein Sequence
LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
82.7kDa
Protein Length
Extracellular Domain
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Recombinant rat neural cell adhesion molecule 1 (Ncam1) production starts with isolating the target gene corresponding to the rat Ncam1 (20-721aa). This gene is co-inserted into an expression vector with an N-terminal 10xHis-tag and C-terminal Myc-tag gene and then introduced into E. coli cells via transformation. The positive E. coli are cultured and induced to express the recombinant Ncam1 protein, which is subsequently harvested from the cell lysate. Purification of the recombinant Ncam1 protein is commonly achieved using affinity chromatography. Its purity is over 90% as determined by SDS-PAGE.

Rat Ncam1 is expressed in different tissues and cell types in rats, including the hippocampus, cardiomyocytes, and pulmonary arterial smooth muscle cells [1][2][3]. In the hippocampus, Ncam1 is associated with adult neurogenesis and is expressed by immature neurons, contributing to cell communication and stem cell functions [4][5]. Ncam1 expression has been linked to cognitive function, with studies indicating that manipulating its expression levels can impact cognitive outcomes in rats [6][7].

Furthermore, Ncam1 has been implicated in synaptic plasticity and neuronal differentiation. Research has demonstrated that Ncam1 isoforms are involved in proteome dynamics during neuronal differentiation, affecting processes such as cell differentiation and growth cone formation. Evidence has found that Ncam1 deficiency disrupts long-term synaptic plasticity, thus impairing the ability of learning and memory.

Ncam1 is associated with postoperative cognitive dysfunction and pulmonary hypertension. Studies have indicated that modulating Ncam1 expression levels can influence cognitive function recovery in rats with postoperative cognitive dysfunction [7].

References:
[1] T. Seki, Hippocampal adult neurogenesis occurs in a microenvironment provided by psa‐ncam‐expressing immature neurons, Journal of Neuroscience Research, vol. 69, no. 6, p. 772-783, 2002. https://doi.org/10.1002/jnr.10366
[2] L. Kahn, G. Alonso, E. Normand, & O. Manzoni, Repeated morphine treatment alters polysialylated neural cell adhesion molecule, glutamate decarboxylase‐67 expression and cell proliferation in the adult rat hippocampus, European Journal of Neuroscience, vol. 21, no. 2, p. 493-500, 2005. https://doi.org/10.1111/j.1460-9568.2005.03883.x
[3] Q. Kong, M. Xia, R. Sun, J. Gao, R. Liang, L. Liet al., Effects of pharmacological treatments on hippocampal ncam1 and erk2 expression in epileptic rats with cognitive dysfunction, Oncology Letters, vol. 12, no. 3, p. 1783-1791, 2016. https://doi.org/10.3892/ol.2016.4882
[4] C. Frese, M. Mikhaylova, R. Stucchi, V. Gautier, Q. Liu, S. Mohammedet al., Quantitative map of proteome dynamics during neuronal differentiation, Cell Reports, vol. 18, no. 6, p. 1527-1542, 2017. https://doi.org/10.1016/j.celrep.2017.01.025
[5] H. Cremer, G. Chazal, A. Carleton, C. Goridis, J. Vincent, & P. Lledo, Long-term but not short-term plasticity at mossy fiber synapses is impaired in neural cell adhesion molecule-deficient mice, Proceedings of the National Academy of Sciences, vol. 95, no. 22, p. 13242-13247, 1998. https://doi.org/10.1073/pnas.95.22.13242
[6] X. Yu, S. Liu, J. Li, X. Fan, Y. Chen, X. Biet al., Microrna-572 improves early post-operative cognitive dysfunction by down-regulating neural cell adhesion molecule 1, Plos One, vol. 10, no. 2, p. e0118511, 2015. https://doi.org/10.1371/journal.pone.0118511
[7] A. Luo, Proteomic and phosphoproteomic analysis of right ventricular hypertrophy in the pulmonary hypertension rat model, Journal of Proteome Research, vol. 23, no. 1, p. 264-276, 2023. https://doi.org/10.1021/acs.jproteome.3c00546

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Target Background

Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Gene References into Functions
  1. Our results strongly support the hypothesis that PSA-NCAMs play a proaggregatory role in the fasciculatory process PMID: 23963795
  2. CD56(140kD) up-regulation plays a pivotal role in the pathogenesis of ischemic cardiomyopathy. PMID: 23462508
  3. Stressed animals exhibited an increase in NCAM mRNA expression in the hippocampus, but not in the prefrontal cortex. The synaptosomal NCAM of the hippocampus was markedly polysialylated, while cortical PSA-NCAM was significantly decreased. PMID: 22814229
  4. A serine-to-alanine exchange at position 774 of NCAM resulted in decreased activation of the cAMP response element binding protein and decreased neurite outgrowth after NCAM stimulation in neuroblastoma cells PMID: 22419107
  5. FGL triggers a long-lasting enhancement of synaptic transmission and long-term potentiation in hippocampal CA1 neurons by a facilitated synaptic delivery of AMPA receptors. PMID: 22363206
  6. NCAM-derived peptide plannexin can protect immature neurons in vivo; however, in a model of status epilepticus plannexin-treated animals do not reveal any neuroprotective effects of plannexin on mature neurons. PMID: 21787839
  7. Treatment with triiodothyronine downregulates expression of NCAM in the seminiferous tubules of rats with flutamide-induced cryptorchidism. PMID: 22007488
  8. Polysialylated-NCAM expression is important for the connectivity of interneurons in the adult cerebral cortex; its regulation may play an important role in the structural plasticity of inhibitory networks. PMID: 20843898
  9. Plannexin facilitates neurite outgrowth in primary hippocampal neuronal cultures and improves spatial learning under conditions involving a deficit in a key plasticity-promoting posttranslational modification of NCAM, its polysialylation. PMID: 21887252
  10. We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling PMID: 21876469
  11. N-CAM is influenced by an intentionally misdirected outgrowth of motor and sensory axons following nerve repair. PMID: 20622691
  12. NCAM is a cardioprotective factor up-regulated under metabolic stress in cardiomyocytes PMID: 19853610
  13. Results indicate that the phosphorylation-dependent interaction between 14-3-3beta and alphaII spectrin acts as a switch between positive and negative regulation of neurite outgrowth stimulated by NCAM. PMID: 20598904
  14. a decisive role for the neuronal K(+) channel in regulating NCAM-dependent neurite outgrowth and attribute a physiologically meaningful role to the functional interplay of Kir3.3, NCAM, and TrkB in ontogeny PMID: 20610389
  15. Kidney cell proliferation, assessed by BrdU and PCNA labeling, was prominent in outer medulla and reach a maximum between 24 and 72 hours after reperfusion. This population was characterized by the coexpression of vimentin and nestin. PMID: 20617137
  16. the activation of FRS2alpha by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor PMID: 20175207
  17. results suggest a positive role of NCAM in neurogenesis in the periventricular region; expression of NCAM in stem cells might be one of many factors useful for therapeutic approaches in the future PMID: 19788570
  18. microdomains responsive to NCAM-mediated signaling from growth cones. PMID: 11817895
  19. distribution of the NCAM immunoreactivity in the sciatic nerves of 10- and 15-day-old Wistar rats PMID: 11936192
  20. Reciprocal actions of neural cell adhesion molecule and tissue plasminogen activator via a Ras-dependent mitogen-activated protein kinase signaling pathway in hippocampal neurons. PMID: 12387826
  21. The effect of exposure to restraint stress on NCAM mRNA was evaluated in the hippocampus. A decrease in NCAM mRNA was found throughout all hippocampal subregions. Stress may thus induce structural alterations in the the hippocampus. PMID: 12453492
  22. Integrin alphav and NCAM mediate the effects of GDNF on DA neuron survival, outgrowth, DA turnover and motor activity in rats. PMID: 12493556
  23. Polysialic acid NCAM expression appears first in the developing inner hair cells (IHCs) of the cochlea and then in the neighboring Kolliker's organ in an increasingly mediolateral gradient. PMID: 12640664
  24. results showed that the protein concentration of the NCAM-140 isoform decreased in the hippocampus of stressed rats PMID: 12757368
  25. Loss or inactivation of the polysialyl tail of NCAM reduces the migration of mechanically wounded oligodendrocyte progenitor-like (O-2A) cells into the injured area and completely blocks the wound closure. PMID: 12774308
  26. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons PMID: 12786978
  27. direct interaction between NCAM and fibroblast growth factor receptor 1 (Ig modules 2 and 3) by surface plasmon resonance analysis PMID: 12791257
  28. Association of NCAM with GFRalpha1 downregulates NCAM-mediated cell adhesion and promotes GDNF binding to NCAM, resulting in rapid activation of cytoplasmic protein tyrosine kinases Fyn and FAK. PMID: 12837245
  29. Strong overexpression of NCAM(CD56) and RUNX1(AML1) is a constant and characteristic feature of cardiomyocytes within or adjacent to scars in ICM. PMID: 12937148
  30. Traumatic fear conditioning suppresses NCAM-PSA-mediated plasticity and the concomitant inability to store the trace of recently acquired information. PMID: 13129654
  31. neural cell adhesion molecule is associated with major components of the cytoskeleton PMID: 14550299
  32. The most intense polysialylated NCAM immunoreactivity is localized in the medial and triangular septal nuclei, regions of the brain that regulate hippocampal theta rhythm during memory consolidation. PMID: 14648585
  33. demonstrated for the first time that IUGR newborn rat kidneys express N-CAM and cadherin adhesion molecules at specific sites of the nephron. PMID: 14663363
  34. Amygdaloid-kindling increases polysialylated NCAM-immunoreactive mossy fibers and their terminals in the stratum lucidum of the hippocampal CA3 region and in the granule cells of the dentate gyrus. PMID: 14741393
  35. The circulating count of CD56(dim) natural killer cells does not change during exercise training. PMID: 14752119
  36. 12 h posttraining, immunohistochemical analyses revealed a learning-induced up-regulation of polysialylated neural cell adhesion molecule in the dentate gyrus related to the spatial learning abilities displayed by rats during training. PMID: 15169853
  37. Relevance of NCAM for tumor cell binding was proven by treating neuroblastoma cells with NCAM antisense oligonucleotides or NCAM transfection. PMID: 15256054
  38. We therefore suggest that early synaptic NCAM expression may be one of the factors determining the capability of rats to learn. PMID: 15490465
  39. Several interacting partners of cytosolic domains of rat brain neural cell adhesion molecule (NCAM) isoforms 140 and 180 have been identified that may play a role in NCAM-mediated signal transduction. PMID: 15865439
  40. Results suggested that the expressions of NCAM, M-cadherin and beta-catenin in skeletal muscle satellite cells may be controlled by distinct regulatory mechanisms during functional overload and muscle hypertrophy. PMID: 16776666
  41. Decreased NCAM-1 expression in CA1 and CA3 fields of the hippocampus, and in a lesser degree in the basal ganglia, limbic structure and cervical spinal cord in autoimmune encephalitis PMID: 17064783
  42. upregulation of NCAM is a correlate of fear conditioning that is not necessary for the establishment of fear memory in the amygdala, but participates in mechanisms precluding fear extinction PMID: 17223582
  43. These findings show evidence of a selective neuronal expression of PSA-NCAM in developing thalamus, supporting its suggested role in cell migration and synaptogenesis as it occurs in the cerebral cortex PMID: 17292800
  44. The upregulation of PSA-NCAM by hyperthermia may have a significant impact on hippocampal plasticity, permitting induction of the complex molecular cascade responsible for neuroprotection. PMID: 17336079
  45. After a dose of a CB1 receptor agonist the number of PSA-NCAM immunoreactive (IR) cells in the dentate gyrus declined in a time-dependent manner. PMID: 17355876
  46. Exposure to stressors during juvenility alters NCAM expression and potentially underlies the predisposition to exhibit stress-related symptoms in adulthood. PMID: 17429411
  47. upregulation of PSA-NCAM expression in the dorsal (but not ventral) dentate gyrus at 24 h after contextual (but not tone) fear conditioning PMID: 17460068
  48. modulation of PSA-NCAM function may promote neuroregeneration in different central nervous system insults PMID: 17513116
  49. Fluoride causes oxidative stress, apoptosis, and decreased mRNA and protein expression levels of NCAM in rat hippocampal neurons. PMID: 17537562
  50. Given the role of PSA-NCAM in neuronal plasticity, the present results indicate that, even during aging, many regions of the CNS may display neurite, spine or synaptic remodeling. PMID: 17904697

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Database Links
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