Recombinant Rat Neurolysin, mitochondrial (Nln)

Code CSB-YP337169RA
MSDS
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Source Yeast
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Code CSB-EP337169RA
MSDS
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Source E.coli
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Code CSB-EP337169RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337169RA
MSDS
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Source Baculovirus
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Code CSB-MP337169RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nln
Uniprot No.
Alternative Names
Nln; Neurolysin; mitochondrial; EC 3.4.24.16; Microsomal endopeptidase; MEP; Mitochondrial oligopeptidase M; Neurotensin endopeptidase
Species
Rattus norvegicus (Rat)
Expression Region
38-704
Target Protein Sequence
SSY TAAGRNVLRW DLSPEQIKTR TEQLIAQTKQ VYDTVGTIAL KEVTYENCLQ VLADIEVTYI VERTMLDFPQ HVSSDREVRA ASTEADKKLS RFDIEMSMRE DVFQRIVHLQ ETCDLEKIKP EARRYLEKSI KMGKRNGLHL SEHIRNEIKS MKKRMSELCI DFNKNLNEDD TSLVFSKAEL GALPDDFIDS LEKTDEDKYK VTLKYPHYFP VMKKCCVPET RRKMEMAFHT RCKQENTAIL QQLLPLRAQV AKLLGYNTHA DFVLELNTAK STSRVAAFLD DLSQKLKPLG EAEREFILSL KKKECEERGF EYDGKINAWD LHYYMTQTEE LKYSVDQESL KEYFPIEVVT EGLLSIYQEL LGLSFEQVPD AHVWNKSVSL YTVKDKATGE VLGQFYLDLY PREGKYNHAA CFGLQPGCLL PDGSRMMSVA ALVVNFSQPV AGRPSLLRHD EVRTYFHEFG HVMHQICAQT DFARFSGTNV ETDFVEVPSQ MLENWVWDVD SLRKLSKHYK DGHPITDELL EKLVASRLVN TGLLTLRQIV LSKVDQSLHT NATLDAASEY AKYCTEILGV AATPGTNMPA TFGHLAGGYD GQYYGYLWSE VFSMDMFHSC FKKEGIMNPE VGMKYRNLIL KPGGSLDGMD MLQNFLQREP NQKAFLMSRG LNGS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.
Gene References into Functions
  1. Data indicate that the inhibitor binds away from the catalytic site of neuropeptidase neurolysin. PMID: 25378390
  2. Neurolysin was identified as a non-AT1 and non-AT2 binding site for Angiotensin II, suggesting that neurolysin may significantly affect the activity of the renin-angiotensin system. PMID: 23412923
  3. this is the first evidence of an in vivo role for EP 24.16 in brain function, specifically in regulating alterations in microvessel permeability induced by exogenous bradykinin PMID: 12586639
  4. EP24.16 activity in vascular endothelial cells may play an important role in the degradation of bradykinin and/or other peptides in the circulation PMID: 12609826
  5. Taken together, these data demonstrate that EP24.15/16 modulate intracellular, peptidergic signaling cascades through B2R in a physiologically relevant nociceptive system. PMID: 16515556
  6. Ciliary epithelium expresses neurolysin. PMID: 17882707

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Subcellular Location
Mitochondrion intermembrane space. Cytoplasm.
Protein Families
Peptidase M3 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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