Recombinant Rat Peroxiredoxin-1 (Prdx1)

Code CSB-YP737225RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP737225RA
MSDS
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Source E.coli
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Code CSB-EP737225RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP737225RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP737225RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Prdx1
Uniprot No.
Alternative Names
Prdx1; Tdpx2Peroxiredoxin-1; EC 1.11.1.15; HBP23; Heme-binding 23 kDa protein; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2
Species
Rattus norvegicus (Rat)
Expression Region
2-199
Target Protein Sequence
SSGNAKIGH PAPSFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWINT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITINDLPVG RSVDEILRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVNK SKEYFSKQK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Gene References into Functions
  1. Prdx1 inhibited MAPK pathway induced cells apoptosis, and reactive oxygen species is the upstream regulator of hypoxia/reoxygenation induced apoptosis. PMID: 29410271
  2. peroxiredoxin 1 has a role in redox sensing and transducing [review] PMID: 27756681
  3. Increased expression of Prdx1 was identified in astrocytes and microglia following traumatic spinal cord injury. PMID: 26003307
  4. Prx1 may alleviate renal tubulointerstitial fibrosis by inhibiting oxidative stress induced apoptosis via p38 MAPK pathway. PMID: 25989822
  5. Prdx 1 expression increased during late gestation & peaked at postnatal-day 1, when its expression gradually decreased. It was strongly expressed in the corpus callosum. PMID: 22166015
  6. peroxiredoxin I gene expression is activated via a protein kinase C, Ras, p38 mitogen-activated protein kinase signaling pathway PMID: 12960165
  7. mammalian sulfiredoxin reactivates hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine PMID: 15448164
  8. the complementary hepatic gene expression pattern of HO-1 and Prx I in response to oxidative stress may suggest a functional interplay of these antioxidant genes PMID: 16309569
  9. hippocampal neurons transfected with wild type Prx1 exhibit increased Abeta resistance PMID: 17761673
  10. X-ray diffraction analysis showed HBP23/Prx I has a Cys(83)-Cys(83) disulfide bond at only one dimer-dimer interface, whereas the interactions at the other interfaces appear to involve hydrophobic and van der Waals forces. PMID: 17974571

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Subcellular Location
Cytoplasm.
Protein Families
Peroxiredoxin family, AhpC/Prx1 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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