Recombinant Rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha(Ppargc1a)

Code CSB-YP889889RA
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Source Yeast
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Code CSB-EP889889RA
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Source E.coli
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Code CSB-EP889889RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889889RA
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Source Baculovirus
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Code CSB-MP889889RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ppargc1a
Uniprot No. Q9QYK2
Alternative Names Ppargc1a; Pgc1; Pgc1a; Ppargc1; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
Species Rattus norvegicus (Rat)
Expression Region 1-796
Target Protein Sequence MAWDMCSQDS VWSDIECAAL VGEDQPLCPD LPELDLSELD VNDLDTDSFL GGLKWCSDQS EIISNQYNNE PANIFEKIDE ENEANLLAVL TETLDSLPVD EDGLPSFDAL TDGDVTTDNE ASPSSMPDGT PPPQEAEEPS LLKKLLLAPA NTQLSYNECS GLSTQNHANH THRIRTNPAI VKTENSWSNK AKSICQQQKP QRRPCSELLK YLTTNDDPPH TKPTENRNSS RDKCASKKKS HTQPQSQHAQ AKPTTLSLPL TPESPNDPKG SPFENKTIER TLSVELSGTA GLTPPTTPPH KANQDNPFKA SPKLKPSCKT VVPPPTKRAR YSECSGTQGS HSTKKGPEQS ELYAQLSKSS VLSRGHEERK TKRPSLRLFG DHDYCQSVNS KTDILINISQ ELQDSRQLDF KDASCDWQGH ICSSTDSSQC YLRETLEASK QVSPCSTRKQ LQDQEIRAEL NKHFGHPSQA VFDDKVDKTS ELRDGNFSNE QFSKLPVFIN SGLAMDGLFD DSEDENDKLS YPWDGTQSYS LFDVSPSCSS FNSPCRDSVS PPKSLFSQRP QRMRSRSRSF SRHRSCSRSP YSRSRSRSPG SRSSSRSCYY YESSHYRHRT HRNSPLYVRS RSRSPYSRRP RYDSYEANEH ERLKRDEYRR EYEKRESERA KQRERQKQKA IEERRVIYVG KIRPDTTRTE LRDRFEVFGE IEECTVNLRD DGDSYGFITY RYTCDAFAAL ENGYTLRRSN ETDFELYFCG RKQFFKSNYA DLDSNSDDFD PASTKSKYDS LDFDSLLKEA QRSLRR
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).
Gene References into Functions
  1. PGC-1alpha knock down also abolished the increased protein level of GLUT4 and decreased the protein level of CPT-1b in cardiomyocytes by chronic intermittent hypobaric hypoxia treatment. PMID: 30396162
  2. the present study demonstrated that NTPGC1alpha alleviated PEinduced mitochondrial impairment and decreased lipid droplet accumulation in NRCMs, indicating that NTPGC1alpha may have ameliorated mitochondrial energy defects in NRCMs, and may be considered as a potential target for the treatment of heart failure. PMID: 29901150
  3. Studied cardioprotective effects of Yiqihuoxue decoction (YQHX) on ischemic conditions both in vivo and vitro. Found YQHX upregulates the PGC-1alpha (PPARG coactivator 1 alpha) expression in MI rats and improves mitochondrial function. PMID: 30223807
  4. PPAR-beta increases PGC-1alpha content via a reduction in protein degradation. PMID: 28467933
  5. In H9c2 cardiomyocyte, it was found that miR-22 mimic aggravated, whilst miR-22 inhibitor significantly attenuated H/R-induced mitochondrial damage. By using real time PCR, western blot and dual-luciferase reporter gene analyses, we identified Sirt1 and PGC1alpha as miR-22 targets in cardiomyocytes PMID: 27174562
  6. Exercise training may inhibit age-induced myonuclear apoptosis by stimulating SIRT1/PGC-1alpha mRNA expression, thereby preventing sarcopenia in aging rat. PMID: 27526155
  7. The accelerated conversion of T4 to T3 within myocytes mediates part of the PGC-1a induction by treadmill exercise and its downstream effects on mitochondrial function PMID: 27302464
  8. Results demonstrated that intraspinal administration of lentiviral vectors significantly increases PGC-1alpha expression, resulting in the inhibition of neuronal apoptotic changes, increased potential of axonal regeneration, and improved motor function in a rat model of traumatic spinal cord injury. Moreover, down-regulation of RhoA-ROCK may partially underlie this neuroprotection. PMID: 27132229
  9. Data suggest that Peroxisome-proliferator-activated receptor co-activator-1alpha protects rat brains via a mitochondrial pathway following intracerebral hemorrhage . PMID: 27178364
  10. CTRP3 modulates mitochondrial energy production through targets of reactive oxygen species and proliferator-activated receptor-gamma coactivator-1alpha in vascular smooth muscle cells PMID: 26844631
  11. PGC-1-alpha inhibits AngII-induced vascular smooth muscle cells proliferation and migration. PMID: 26310573
  12. Dihydromyricetin can stimulate irisin secretion partially via the PGC-1alpha pathway. PMID: 26054747
  13. These findings suggest that the statin-induced activation of muscle atrophy genes occurs independent of changes in PGC-1alpha protein and mitochondrial content. PMID: 26020641
  14. The data suggest that PGC-1alpha may protect diabetic nephropathy via the inhibition of DRP1-mediated mitochondrial dynamic remodeling and reactive oxygen species production. PMID: 25853493
  15. Data suggest that expression of Ppargc1a can be regulated by dietary factors; here, dietary flavones (as nootropic dietary supplements) up-regulate expression of Ppargc1a in hippocampus and prevent memory deficit in a model of Alzheimer disease. PMID: 25972379
  16. binding activity and transcription activity of NFATc4 to DNA promoter of brain natriuretic peptide were abrogated by PGC-1alpha overexpression but were enhanced by PGC-1alpha knockdown PMID: 26118953
  17. PPARGC1a has a role in intrauterine growth restriction in a rat model that involves insulin resistance PMID: 25675425
  18. Modest PGC-1alpha protein ablation resulted in decreased mitochondrial activity in general and FAs oxidation in particular without increased FAs cellular influx. PMID: 25824459
  19. ACh activates SOD2 within mitochondria through FoxO3a/PGC-1alpha pathway. PMID: 25402826
  20. SIRT1 activation may alleviate mitochondrial oxidative stress induced by seizures partially via PGC-1alpha signaling. PMID: 25333348
  21. long-term BPA exposure induces cardiomyopathy in male rats; the underlying mechanism might involve the impairment of cardiac mitochondrial function and the disturbance of PGC-1alpha methylation PMID: 25572651
  22. PGC-1alpha is differentially expressed during aseptic inflammation and infection and it is necessary for adequate phagocytosis PMID: 24562467
  23. Gene expression of peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha), which is a master regulator of mitochondrial biogenesis, was decreased in the hippocamus of gonadectomized rats. PMID: 24005768
  24. Significant overexpression of PGC1-alpha in the stress-subjected rats. PMID: 23271280
  25. Brain aging was associated with an imbalance between the PI3K/Akt pathway of insulin signaling and c-Jun N-terminal kinase (JNK) signaling and a downregulation of the PGC1alpha-mediated transcriptional pathway. PMID: 23815272
  26. Results support the idea that stimulation of PGC1alpha represents a potential regulatory factor for ventricular oxidative metabolism after myocardial infarct. PMID: 23880578
  27. Activation of the PGC-1alpha/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in beta-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation after cold exposure. PMID: 23948478
  28. endurance training can attenuate aging-associated decline in mitochondrial protein synthesis in skeletal muscle partly due to upregulation of PGC-1alpha signaling PMID: 23994518
  29. Data indicate that pre-diabetes state decreased both testicular peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1alpha) and Sirtuin 3 (Sirt3). PMID: 24361842
  30. Maternal protein restriction in rats leads to reduced PGC-1alpha expression via altered DNA methylation in skeletal muscle. PMID: 23117952
  31. Following excitotoxicity and neuroinflammation induced by lipopolysaccharide there is an increase in PGC-1alpha in neonatal rat brain. PMID: 23635713
  32. PGC-1alpha is a potent target for aluminium neurotoxicity, which makes it an almost ideal target to control or limit the damage that has been associated with the defective mitochondrial function seen in neurodegenerative diseases. PMID: 24084166
  33. Following ischemic-reperfusion injury in the kidney, PGC-1alpha is activated following treatment with a SIRT1 activator. PMID: 24096033
  34. Gly482Ser mutation blunts the effect of PGC-1alpha overexpression on stimulating hepatocyte CPT1a expression. PMID: 23602251
  35. Collectively, these findings showed that high glucose promoted apoptosis and cell growth arrest in podocytes via IL-6 signaling. PMID: 23692924
  36. Our data demonstrate that depletion of oxygen or fuel results in repression or induction, respectively, of PGC-1alpha expression PMID: 23515531
  37. Results suggest that PGC-1alpha might protect cochlear marginal cells in this cell model from senescence through a nuclear-mitochondrial interaction and against apoptosis. PMID: 23159434
  38. role in formation and maintenance of neuronal dendritic spines PMID: 23212379
  39. Both Ppargc1-alpha and hypoxia induced factor (HIF)1-alpha play crucial roles in hypoxic preconditioning in endothelial and neuronal cells. PMID: 22342160
  40. Acetylcholine protected against deficient mitochondrial biogenesis and function in myocardial reperfusion injury in a cellular model through muscarinic receptor-mediated, AMPK/PGC-1alpha-associated regulatory program. PMID: 23139024
  41. overexpression of hepatic PGC-1alpha and subsequent increases in fatty acid oxidation through elevated mitochondrial content and/or function result in reduced triacylglyceride storage and secretion in the in vitro and in vivo milieu. PMID: 22899824
  42. RIP140 and PGC-1alpha exert an antagonistic role in regulating cardiac energy state and mitochondrial biogenesis. PMID: 22503866
  43. PGC-1alpha is a key regulator mediating mitochondrial biogenesis and the proliferation of pulmonary artery vascular smooth muscle cells at an early stage of hypoxic exposure. PMID: 22527940
  44. the low mitochondrial biogenesis associated with aging may be due to the lack of response of PGC-1alpha to different stimuli. PMID: 21590341
  45. Found overexpression of PGC-1alpha attenuated the proliferation, migration and inflammatory effects of glucose and decreased mRNA and protein level of VSMCs-related genes, and induced macrophage-related gene expression, as well as phagocytosis of VSMCs. PMID: 22461724
  46. Acetyl-L-carnitine activates the peroxisome proliferator-activated receptor-gamma coactivators PGC-1alpha/PGC-1beta-dependent signaling cascade of mitochondrial biogenesis and decreases the oxidized peroxiredoxins content in old rat liver PMID: 22533417
  47. The ability of epinephrine and AICAR to induce PGC-1alpha remains intact in skeletal muscle from high fat diet-fed rats. PMID: 22496244
  48. knock-down of endogenous PGC-1a increased NMDAREX activity and vulnerability to excitotoxic insults in rat cortical neurons. In contrast, exogenous expression of PGC-1a resulted in a neuroprotective reduction of NMDAREX. PMID: 22593067
  49. Unlike our previous observations in pancreatic beta-cell mass, there was no "reprogramming" effect of early exercise on adult skeletal muscle such that PGC-1alpha was lower in adult Restricted and Reduced litter offspring. PMID: 22354784
  50. effects of running exercise on fiber characteristics, oxidative capacity, and PGC-1alpha mRNA levels in the soleus muscles of metabolic syndrome [SHR/NDmcr-cp (cp/cp PMID: 22234788
  51. The PGC-1alpha is antagonistic regulators of neuronal vulnerability to oxidative stress. PMID: 20849372
  52. Status epilepticus (SE) can induce the PGC-1alpha/mitochondrial antioxidant system signaling pathway, which may represent a protective mechanism against SE-induced oxidative stress. PMID: 21536107
  53. Conclude that decreased oxidative capacity and fiber atrophy in unloaded skeletal muscles are associated with decreased PGC-1alpha and increased FOXO1 mRNA levels. PMID: 21972093
  54. It would appear that increased mitochondrial biogenesis known to occur in copper deficiency hearts is caused by an increased expression in the master regulator of mitochondrial biogenesis, PGC-1alpha. PMID: 18993053
  55. IHG-1 increases mitochondrial biogenesis by promoting PGC-1alpha-dependent processes, potentially contributing to the pathogenesis of renal fibrosis PMID: 21784897
  56. The elevation of plasma FFA levels may induce insulin resistance and decreases the mRNA expression of PGC-1alpha in skeletal muscle. PMID: 21162799
  57. PGC-1alpha seems to act as a regulator of metabolism and/or survival in GABAergic neurons during a phase of mitochondrial and synaptic changes in the developing brain [peroxisome proliferator activated receptor gamma, coactivator 1alpha] PMID: 17335037
  58. Study provides correlative evidence for the neuroprotective cascade of calcium/calmodulin-dependent protein kinase IV/Ppargc1alpha in delayed neuronal cell death and mitochondrial biogenesis in the hippocampus PMID: 20799369
  59. The PGC-1alpha content in nuclear fractions in the muscle and ALAS mRNA was higher than that of the control rats' muscle by 58% and 25%. PMID: 21307617
  60. Activation of AMPK increases PGC-1alpha mRNA levels in visual cortex neuron cultures. 1 week of monocular deprivation significantly reduces AMPK phosphorylation & down-regulates PGC-1alpha expression in deprived primary visual cortex. PMID: 20438809
  61. Data demonstrate that calcineurin activity is a major determinant of PGC-1alpha expression in skeletal muscle during diabetes and possibly other conditions associated with loss of muscle mass. PMID: 20359506
  62. results suggest that down-expression of PGC-1alpha partially mediated by activation of JNK/c-Jun may be through the binding of c-Jun to the CRE site in the PGC-1alpha promoter, and it might be involved in cerebellar granule neuron apoptosis PMID: 20143420
  63. These data suggest that PGC-1 alpha can ameliorate cardiac Ca(2+) cycling and improve cardiac work output in response to physiological stress. PMID: 20457122
  64. Data show that in oleic acid (OA)-stimulated rat vascular smooth muscle cells (VSMCs), treatment with estradiol diminishes VSMC proliferation and migration via restoring OA-decreased PGC-1alpha expression. PMID: 19786068
  65. Knockdown of Pgc-1 in cultured rat granulosa cells resulted in attenuation of gene expression as well as progesterone production. PMID: 20133449
  66. p38 MAPK and calcium channels mediate the signaling mechanism in activating PGC-1 in neurons. The close correlation between message and protein levels indicates that the regulation of PGC-1 expression in neurons is primarily at the transcriptional level. PMID: 19774670
  67. endogenous PGC-1 alpha is a negative regulator of vascular hypertrophy by up-regulating catalase expression and thus reducing reactive oxygen species levels PMID: 19940161
  68. T(3) regulates PGC-1 alpha abundance and association with hepatic genes, and in turn PGC-1 alpha is an important participant in the T(3) induction of selected genes PMID: 19948729
  69. proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) emerges as a potential transcriptional regulator of the inflammatory phenomenon taking place in the steatotic liver PMID: 19766270
  70. The contraction-activated PGC-1alpha signaling pathways in skeletal muscle are redox sensitive and that nonmitochondrial ROS play an important role in stimulating mitochondrial biogenesis. PMID: 19686839
  71. Peroxisome proliferator-activated receptor (PPAR) gamma coactivator-1 recruitment regulates PPAR subtype specificity. PMID: 11875072
  72. increased PGC-1 expression and subsequent hepatic glucose production contribute to the insulin resistance observed in the intrauterine growth retardation juvenile rat PMID: 12072378
  73. Results suggest that an acute bout of low-intensity prolonged exercise directly enhances PGC-1 mRNA expression in the activated muscle, possibly through a 5'AMP-activated protein kinase-related mechanism. PMID: 12163024
  74. PGC-1alpha is widely expressed in the rodent brain, but is not regulated by states of caloric deficiency, leptin, obesity or cold exposure PMID: 12531492
  75. Peroxisome proliferator-activated receptor (PPAR) alpha and PPARbeta/delta, but not PPARgamma, modulate the expression of genes involved in cardiac lipid metabolism. PMID: 12600885
  76. Data suggest that increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) expression form part of a unifying pathway that promotes both triiodothyronine- and contractile activity-induced mitochondrial adaptations. PMID: 12734114
  77. Rev-Erbalpha as a target gene of PPARgamma in adipose tissue and demonstrate a role for this nuclear receptor as a promoter of adipocyte differentiation. PMID: 12821652
  78. Exercise-induced PGC-1alpha expression in skeletal muscle may be mediated by at least two exercise-induced signaling factors: AMPK activation and Ca2+ elevation. PMID: 14570700
  79. Sympathetic sympathetic tonus generated by exposure of rats to cold induces the expression of PGC-1. PMID: 14576981
  80. markedly amplifies PEPCK gene expression and gluconeogenesis PMID: 15044597
  81. cold-induced hyperexpression of PGC-1alpha participates in control of skeletal muscle glucose uptake through a mechanism that controls GLUT4 expression PMID: 15165993
  82. SIRT1 catalyzes PGC-1alpha deacetylation both in vitro and in vivo PMID: 15716268
  83. elevated PGC-1alpha by free fatty acids plays an important role in chronic hyperlipidemia-induced beta-cell dysfunction PMID: 15733855
  84. activation of pyruvate dehydrogenase kinase 4 by the peroxisome proliferator-activated receptor gamma coactivator. PMID: 15967803
  85. results demonstrate that protein kinase LKB1 and Peroxisome-proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) abundances increase with endurance and interval training PMID: 16014350
  86. These results suggest that lipopolysaccharide-mediated NF-kappaB activation inhibits the expression of genes involved in fatty acid metabolism by a mechanism involving reduced expression of PGC-1. PMID: 16139565
  87. Results show that both PGC-1alpha and PGC-1beta are involved in the regulation of skeletal muscle fiber transition and metabolism and that they have both overlapping and differing effects. PMID: 16720625
  88. activation of PGC-1alpha may mediate the initial phase of the exercise-induced adaptive increase in muscle mitochondria, whereas the subsequent increase in PGC-1alpha protein sustains and enhances the increase in mitochondrial biogenesis PMID: 17099248
  89. Peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha) and protein kinase C-zeta (PKC-zeta), are involved in the regulation of muscle glucose transport. PMID: 17284668
  90. p38 mitogen-activated protein kinase is downstream of calcium/calmodulin-dependent protein kinase in a signaling pathway by which increases in cytosolic calcium lead to increases in ppargc1a expression and mitochondrial biogenesis in muscl PMID: 17488713
  91. calcium signalling is involved in regulating the PGC-1alpha and hexokinase II genes, but not pyruvate dehydrogenase kinase 4 PMID: 17516843
  92. PGC-1alpha responds very early to increased neuronal activity by synthesizing more proteins in the cytoplasm and translocating them to the nucleus for gene activation. PGC-1alpha level in neurons is, therefore, tightly regulated by neuronal activity. PMID: 17870059
  93. endurance exercise does not increase the skeletal muscle PGC-1alpha protein expression PMID: 18555842
  94. Promotes transcription of the manganese-dependent superoxide dismutase, uncoupling protein-2, and an array of other proteins which provide antioxidant protection to mitochondria and may impact the aging process. PMID: 18789599
  95. Exercise and adrenaline increase PGC-1alpha mRNA expression in rat adipose tissue. PMID: 19221126
  96. PGC-1 inhibits insulin and BETA2/NeuroD transcription levels and attenuating PGC-1 overexpression protects against glucolipotoxicity-induced beta-cell dysfunction both in vivo and in vitro. PMID: 19520786
  97. PGC-1{alpha} and PGC-1{beta} regulate mitochondrial density in neurons PMID: 19542216
  98. These data strongly suggest that PGC-1alpha inhibits vascular smooth muscle cell migration and neointimal formation after vascular injury in rats, mainly by upregulating the expression of the mitochondrial antioxidant enzyme SOD-2. PMID: 19553562
  99. NT-PGC-1alpha is a co-expressed, previously unrecognized form of PGC-1alpha with functions that are both unique from and complementary to PGC-1alpha PMID: 19773550

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Subcellular Location Nucleus, Nucleus, PML body
Database Links

KEGG: rno:83516

STRING: 10116.ENSRNOP00000006071

UniGene: Rn.19172

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