Recombinant Rat Probable ATP-dependent RNA helicase DDX4 (Ddx4)

Code CSB-YP720640RA
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Source Yeast
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Code CSB-EP720640RA
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Source E.coli
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Code CSB-EP720640RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP720640RA
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Source Baculovirus
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Code CSB-MP720640RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ddx4
Uniprot No.
Alternative Names
Ddx4Probable ATP-dependent RNA helicase DDX4; EC 3.6.4.13; DEAD box protein 4; Vasa homolog; rVLG
Species
Rattus norvegicus (Rat)
Expression Region
1-713
Target Protein Sequence
MGDEDWEAEI LKPHVSSYVP VFEKDKYSSG ANGDTFNRTS ASSSEMEDGP SGRDHFMRSG FSSGRNLGNR DIGESSKRET TSTTGGFGRG KGFGNRGFLN NKFEEGDSSG FWKESTNDCE DTQTRSRGFS KRGGYPDGND SEASGPFRRG GRDSEYDQDQ GSQRGGGLFG SRKPAASDSG SGDTFQSRSG NARGAYKGLN EEVVTGSGKN SWKSEAEGGE SSDIQGPKVT YIPPPPPEDE DSIFAHYQTG INFDKYDTIL VEVSGHDAPP AILTFEEANL CQTLNNNIAK AGYTKLTPVQ KYSIPIVLAG RDLMACAQTG SGKTAAFLLP ILAHMMRDGI TASRFKELQE PECIIVAPTR ELINQIYLEA RKFSFGTCVR AVVIYGGTQF GHSIRQIVQG CNILCATPGR LMDIIGKEKI GLKQVKYLVL DEADRMLDMG FGPEMKKLIS CPGMPSKEQR QTLLFSATFP EEIQRLAGEF LKSNYLFVAV GQVGGACRDV QQSILQVGPV FKKRKLVEIL RNIGDERPMV FVETKKKADF IATFLCQEKI STTSIHGDRE QREREQALGD FRCGKCPVLV ATSVAARGLD IENVQHVINF NLPSTIDEYV HRIGRTGRCG NTGRAISFFD TESDNHLAQP LVKVLSDAQQ DVPAWLEEIA FSSYAPPSFS NSTRGAVFAS FDTRKNFQGK NTLNTAGISS AQAPNPVDDE SWD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
Subcellular Location
Cytoplasm. Cytoplasm, perinuclear region.
Protein Families
DEAD box helicase family, DDX4/VASA subfamily
Tissue Specificity
Testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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