Recombinant Rat Regulating synaptic membrane exocytosis protein 1 (Rims1), partial

Code CSB-YP881310RA
MSDS
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Source Yeast
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Code CSB-EP881310RA
MSDS
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Source E.coli
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Code CSB-EP881310RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881310RA
MSDS
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Source Baculovirus
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Code CSB-MP881310RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rims1
Uniprot No.
Alternative Names
Rims1; Rim1; Regulating synaptic membrane exocytosis protein 1; Rab-3-interacting molecule 1; RIM 1
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Rab effector involved in exocytosis. May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity. Plays a role in dendrite formation by melanocytes.
Gene References into Functions
  1. RIM1alpha SUMOylation at K502 is required for its binding to Cav2.1 and for fast synaptic vesicle exocytosis. PMID: 24290762
  2. RIM1- or Bassoon-binding region of CAST impairs synaptic transmission in cultured superior cervical ganglion neurons. PMID: 14734538
  3. These data demonstrate that alpha-RIMs are not essential for synapse formation or synaptic exocytosis, but are required for normal Ca(2+)-triggering of exocytosis. PMID: 17124501
  4. The crystal structure of the RIM1alpha C2B domain dimer reveals that it forms a beta-sandwich and that the unique N- and C-terminal extensions form a small subdomain that packs against the beta-sandwich and mediates dimerization. PMID: 17630786
Subcellular Location
Cell membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein. Note=Associated with plasma membranes from synaptic junctions. Not detected in synaptic vesicles. Detected in presynaptic nerve terminals close to the active zone. Detected in synaptic ribbons of ribbon synapses of retinal photoreceptor cells.
Tissue Specificity
Highly expressed in hippocampus, brain cortex, cerebellum and olfactory bulb. Detected at lower levels in midbrain, hindbrain and spinal cord. Detected retina and in spinal cord motor neurons.
Database Links

KEGG: rno:84556

UniGene: Rn.10799

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