Recombinant Rat Rhodopsin (Rho), partial

Code CSB-YP019681RA1
MSDS
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Source Yeast
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Code CSB-EP019681RA1
MSDS
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Source E.coli
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Code CSB-EP019681RA1-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019681RA1
MSDS
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Source Baculovirus
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Code CSB-MP019681RA1
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rho
Uniprot No.
Alternative Names
Rho; Rhodopsin
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
Gene References into Functions
  1. these results suggest that retinoid metabolism in the eye is impaired in type 1 diabetes, which leads to deficient generation of visual pigments and neural retinal dysfunction in early diabetes. PMID: 28734946
  2. EGF induces Ca(2+) sensitization in vascular smooth muscle by Rho-kinase-dependent inactivation of MLCP mediated by the EGF receptor/MEK/Erk1/2 pathway PMID: 26004531
  3. Allele-specific ASO-mediated knockdown of mutant P23H rhodopsin expression slowed the rate of photoreceptor degeneration and preserved the function of photoreceptor cells in eyes of the P23H rhodopsin transgenic rat. PMID: 26436889
  4. Phosphorylation of the Rho/ROCK signaling target cofilin. PMID: 25108566
  5. We examine and compare the contribution of endoplasmic reticulum stress to retinal degeneration in several vertebrate models of retinitis pigementosa generated through expression of mutant rhodopsins. PMID: 24664747
  6. Rho kinase is involved in endothelial dysfunction in type 1 diabetes. PMID: 24129169
  7. The aim of this study was to examine the onset of Unfolded Protein Response (UPR) gene expression in S334ter Rho retinas to determine if UPR is activated in autosomal dominant retinitis pigmentosa animal models. PMID: 22432009
  8. Strong activation of calpain and poly(ADP-ribose) polymerase (PARP) was found in rhodopsin mutants. PMID: 21765948
  9. Phosphorylation of rhodopsin during dark adaptation in an animal model of retinitis pigmentosa. PMID: 12965217
  10. rats homozygous for P23H rhodopsin mutations have severe loss of rod function already seen by postnatal day 21 PMID: 15911114
  11. Insulin receptor phosphorylation in rod photoreceptors is signaled through the G-protein-coupled receptor rhodopsin. PMID: 17272282
  12. Experiments with dissociated hippocampal cells show that the main factor limiting the spatial resolution is Rhodopsin current density rather than scattering of the excitation light. PMID: 18654856

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Subcellular Location
Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment.
Protein Families
G-protein coupled receptor 1 family, Opsin subfamily
Database Links
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