Recombinant Rat SH3 and multiple ankyrin repeat domains protein 1 (Shank1), partial

Code CSB-YP893897RA
MSDS
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Source Yeast
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Code CSB-EP893897RA
MSDS
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Source E.coli
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Code CSB-EP893897RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893897RA
MSDS
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Source Baculovirus
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Code CSB-MP893897RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Shank1
Uniprot No.
Alternative Names
Shank1; SH3 and multiple ankyrin repeat domains protein 1; Shank1; GKAP/SAPAP-interacting protein; SPANK-1; Somatostatin receptor-interacting protein; SSTR-interacting protein; SSTRIP; Synamon
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function.
Gene References into Functions
  1. All three Shank mRNA isoforms are differentially expressed in the synaptic neuropil. PMID: 24298140
  2. this study provides a new level of understanding of the specificity and structural plasticity of the shank PDZ domain. PMID: 21376703
  3. Despite the fact that the betaPIX coiled-coil domain exposes three PDZ binding motifs in the C-termini, the betaPIX trimer associates with a single Shank PDZ. PMID: 20117114
  4. PDZ domain of Shank1 and its complex with the C-terminal octapeptide of GKAP were crystallized PMID: 12136153
  5. the rat shank1 PDZ-ligand complex has a class I PDZ interaction and a novel PDZ-PDZ dimerization PMID: 12954649
  6. The appearance of dendritic Shank mRNAs in cerebellar Purkinje cells coincides with the onset of dendrite formation. Expression of reporter transcripts in hippocampal neurons identifies a 200-nucleotide dendritic targeting element in Shank1 mRNA. PMID: 15121189
  7. Results show that dendritic targeting of shank1 mRNA granules involves KIF5C and the KIF5-associated RNA-binding protein staufen1. PMID: 19416473
  8. Promotes the maturation of dendritic spines and the enlargement of spine heads. Postsynaptic expression of Shank also enhances presynaptic function. PMID: 11498055

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Subcellular Location
Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Note=Colocalizes with alpha-latrotoxin receptor 1.
Protein Families
SHANK family
Tissue Specificity
Expressed only in brain (neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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