Recombinant Rat Signal transducer and activator of transcription 5B(Stat5b)

Code CSB-YP022815RA
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Source Yeast
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Code CSB-EP022815RA
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Source E.coli
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Code CSB-EP022815RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022815RA
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Source Baculovirus
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Code CSB-MP022815RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Stat5b
Uniprot No. P52632
Alternative Names Stat5b; Signal transducer and activator of transcription 5B
Species Rattus norvegicus (Rat)
Expression Region 1-786
Target Protein Sequence MAMWIQAQQL QGDALHQMQA LYGQHFPIEV RHYLSQWIES QAWDSIDLDN PQENIKATQL LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQNTYDR CPMELVRCIR HILYNEQRLV REANNGSSPA GSLADAMSQK HLQINQTFEE LRLITQDTES ELKKLQQTQE YFIIQYQESL RIQAQFAQLA QLNPQERMSR ETALQQKQVS LETWLQREAQ TLQQYRVELA EKHQKTLQLL RKQQTIILDD ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRLEHLC QQLPIPGPVE EMLAEVNATI TDIISALSTS TFIIEKQPPQ VLKTQTKFAA TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN DYSGEILNNC CVMEYHQATG TLSAHFRNMS LKRIKRSDRR GAESVTEEKF TILFDSQFSV GGNELVFQVK TLSLPVVVIV HGSQDNNATA TVLWDNAFRE PGRVPFAVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNS SSNHLEDYNS MSVSWSQFNR ENLPGRNYTF WQWFDGVMEV LKKHLKPHWN DGAILGFVNK QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSQERMF WNLMPFTTRD FSIRSLADRL GDLNYLIYVF PDRPKDEVYS KYYTPVPCER ATAKAADGYV KPQIKQVVPE FVNASTDAGS GATYMDQAPS PVVCPQAHYN MYPQNPDSVL DTDGDFDLED TMDVARRVEE LLGRPMDSQW IPHAQS
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.
Gene References into Functions
  1. Combinatorial interplay among multiple Stat5b-binding response elements with distinguishable biochemical properties is responsible for highly regulated control of IGF-I gene activity by growth hormone. PMID: 23185594
  2. STAT5b plays an essential role in growth hormone signaling activation and osteoblast differentiation. PMID: 23071812
  3. the inhibition of Stat5b expression in chondrocytes prevented the GH promoting effects on NF-kappaB-DNA binding, whereas the inhibition of NF-kappaB p65 expression or activity prevented the GH-dependent activation of IGF-1 and bmp2 expression. PMID: 21592969
  4. GH stimulates recruitment of Stat5b to multiple dispersed regions within the IGFI locus PMID: 20378540
  5. Insulin-like growth factor-I gene transcription is controlled by growth hormone through this protein PMID: 12682066
  6. EGF induces tyrosine phosphorylation and DNA-binding activity of Stat5b in a manner different from GH and PRL, apparently by a Src-dependent mechanism PMID: 12767047
  7. two intronic Stat5 DNA elements were able to bind Stat5b in vitro after GH treatment and could transmit GH- and Stat5b-dependent transcriptional responsiveness to the major IGF-I promoter PMID: 14532269
  8. These results, in addition to establishing STAT5b as one of the key agents of GH-stimulated gene transcription, demonstrate the feasibility of using in vivo gene transfer to target the functions of hormone-activated signal transduction pathways. PMID: 14761873
  9. phosphorylated Stat5b protein increases upon EPO stimulation in Leydig cells PMID: 15062567
  10. The activation of STAT5B by prolactin was biphasic, with an initial peak within 30 min, a nadir between 1 and 3 h, and prolonged activation after 4 h, in contrast, the response to growth hormone was transient for 1 h. PMID: 15142985
  11. growth hormone, via the coordinated action of HNFs and STAT5b, regulates sexually dimorphic liver gene expression [review] PMID: 15253383
  12. Stat5b A630P mutant is an inactive transcription factor by virtue of its aberrant folding and diminished solubility triggered by a misfolded SH2 domain. PMID: 16303763
  13. specific Stat5b binding sites mediate growth hormone-stimulated IGF-I gene transcription PMID: 16339156
  14. STAT-5B/STAT-3/Hsp27/FGF-2 signaling via its involvement in the regulation of vascular smooth muscle growth growth and motility may play an important role in the pathogenesis of vascular disease. PMID: 16527988
  15. around the end of pregnancy, prolactin loses the ability to activate STAT5b PMID: 16857756
  16. suppressor of cytokine signaling 2 is a direct signal transducer and activator of transcription 5b target in liver PMID: 17008382
  17. The cellular actions of GH normally mediated by activation of the Jak2/Stat5B pathway are suppressed in female cells. PMID: 17332060
  18. Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal development, whereas in the adult it decreased. PMID: 17565389
  19. STAT2/5a/5b/6 were confirmed to be significantly modulated during focal cerebral ischaemia. PMID: 17880360

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Subcellular Location Cytoplasm. Nucleus.
Protein Families Transcription factor STAT family
Database Links

KEGG: rno:25126

STRING: 10116.ENSRNOP00000026354

UniGene: Rn.54486

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