Recombinant Rat Transcription factor E2-alpha (Tcf3)

Code CSB-YP023301RA
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Source Yeast
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Code CSB-EP023301RA
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Source E.coli
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Code CSB-EP023301RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023301RA
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Source Baculovirus
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Code CSB-MP023301RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Tcf3
Uniprot No.
Alternative Names
Tcf3; Pan; Ptf1c; Tcfe2a; Transcription factor E2-alpha; Immunoglobulin enhancer-binding factor E12/E47; Pancreas specific transcription factor 1c; Transcription factor 3; TCF-3; Transcription regulator Pan
Species
Rattus norvegicus (Rat)
Expression Region
1-649
Target Protein Sequence
MMNQSQRMAP VGSDKELSDL LDFSMMFPLP VANGKGRPAS LAGTQFAGSG LEDRPSSESW GNSEQNSSSF DPSRAYSEGA HFSDSHSSLP PSTFLGAGLG GKGSERNAYA TFGRDTSVGT LSQAGFLPGE LGLSSPGPLS PSGVKSSSQY YTSFPSNPRR RAADGGLDTQ PKKVRKVPPG LPSSVYPSSS GDNYSRDATA YPSAKTPSSA YPSPFYVADG SLHPSAELWS PPGQVGFGPM LGDGSAPLPL APGSSSVSSG AFGGLQQQDR MGYQLHGSEV NGTLPAVSSF SAAPGTYSGT SGHTPPVSGA DSLLGTRGTT ASSSGDALGK ALASIYSPDH SSNNFSPSPS TPVGSPQGLP GTSQWPRAGA PSALSPNYDA GLHGLSKMED RLDEAIHVLR SHAVGTASEL HGLLPGHSTL TTSFAGPMSL GGRHAGLVSG SHPEDGLTSG ASLLHNHASL PSQPSSLPDL SQRPPDSFSG LGRAGVTAGA SEIKREEKED EEVTSVADAE EDKKDLKVPR TRTSPDEDED DLLPPEQKAE REKERRVANN ARERLRVRDI NEAFKELGRM CQLHLSTEKP QTKLLILHQA VAVILSLEQQ VRERNLNPKA ACLKRREEEK VSGVVGDPQL ALSAAHPGLG EAHNPAGHL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to the consensus sequence CAC/GCTGT/C present, in the chymotrypsin, insulin, AP-4, and several other gene enhancer motifs.
Subcellular Location
Nucleus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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