Recombinant Rat WD repeat domain phosphoinositide-interacting protein 2(Wipi2)

Code CSB-YP724507RA
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP724507RA
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP724507RA-B
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP724507RA
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP724507RA
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity >85% (SDS-PAGE)
Target Names Wipi2
Uniprot No. Q6AY57
Alternative Names Wipi2; WD repeat domain phosphoinositide-interacting protein 2; WIPI-2
Species Rattus norvegicus (Rat)
Expression Region 1-445
Target Protein Sequence MNLASQSGEA GAGQLLFANF NQDNTSLAVG SKSGYKFFSL SSVDKLEQIY ECTDTEDVCI VERLFSSSLV AIVSLKAPRK LKVCHFKKGT EICNYSYSNT ILAVKLNRQR LIVCLEESLY IHNIRDMKVL HTIRETPPNP AGLCALSINN DNCYLAYPGS ATIGEVQVFD TINLRAANMI PAHDSPLAAL AFDASGTKLA TASEKGTVIR VFSIPEGQKL FEFRRGVKRC VSICSLAFSM DGMFLSASSN TETVHIFKLE AVREKPPEEP TTWTGYFGKV LMASTSYLPS QVTEMFNQGR AFATVRLPFC GHKNICSLTT IQKIPRLLVG ASDGYLYMYN LDPQEGGECA LMRQHRLDGS METTSEIVDS ASHDCPLVTQ TYGTAAAKGA YVPSSPTRLG KGQDANLEAY TDDLGAVGGA CLEDEASALR LDEDSEHPPM ILRTD
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.
Subcellular Location Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families WD repeat SVP1 family
Database Links

KEGG: rno:288498

STRING: 10116.ENSRNOP00000001476

UniGene: Rn.163194

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1