Recombinant Rat Wnt inhibitory factor 1 (Wif1)

Code CSB-YP026113RA
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Source Yeast
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Code CSB-EP026113RA
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Source E.coli
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Code CSB-EP026113RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026113RA
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Source Baculovirus
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Code CSB-MP026113RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Wif1
Uniprot No.
Alternative Names
Wif1Wnt inhibitory factor 1; WIF-1
Species
Rattus norvegicus (Rat)
Expression Region
29-379
Target Protein Sequence
GQ PPEESLYLWI DAHQARVLIG FEEDILIVSE GKMAPFTHDF RKAQQRMPAI PVNIHSMNFT WQASGQAEYF YEFLSLRSLD KGIMADPTVN VPRLGTVPHK ASVVQVGFPC LGKQDGVAAF EVNVIVMNSE GNPILRTPQN AIFFKTCQQA ECPGGCRNGG FCNERRVCEC PDGFYGPHCE KALCIPRCMN GGLCVTPGFC ICPPGFYGVN CDKANCSATC FNGGTCFYPG KCICPPGLEG EQCELSKCPQ PCRNGGKCIG KSKCKCPKGY QGDLCSKPVC EPGCGAHGTC HEPNKCQCRE GWHGRHCNKR YGASLMHAPR PAGAGLERHT PSLKKAEGRR DPPESNYIW
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Gene References into Functions
  1. Data show that microRNA miR-17-5p over-expression promoted the activation of hepatic stellate cell (HSC) and contributed to the reduction of Wnt inhibitory factor 1 (WIF1) levels. PMID: 26637809
  2. GLT mRNA was translated in Wnt-1-expressing C6 cells when the GLT mRNA required no splicing before translation occurred PMID: 12470708
  3. Wif-1 inhibits proliferation of neoplastic choroid plexus epithelial cells PMID: 19621428
Subcellular Location
Secreted.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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