Recombinant Rat cGMP-inhibited 3',5'-cyclic phosphodiesterase B (Pde3b), partial

Code CSB-YP730873RA
MSDS
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Source Yeast
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Code CSB-EP730873RA
MSDS
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Source E.coli
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Code CSB-EP730873RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730873RA
MSDS
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Source Baculovirus
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Code CSB-MP730873RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pde3b
Uniprot No.
Alternative Names
Pde3bcGMP-inhibited 3',5'-cyclic phosphodiesterase B; EC 3.1.4.17; CGIPDE1; Cyclic GMP-inhibited phosphodiesterase B; CGI-PDE B
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.
Gene References into Functions
  1. C-peptide has the potential to act, conditionally, as an antilipolytic hormone by activating phosphodiesterase-3B. PMID: 22990990
  2. Data demonstrate that AG/UAG attenuation of ISO-induced lipolysis involves PI3Kgamma/AKT and PDE3B. PMID: 21435395
  3. PDE3B is activated in response to agents relevant for beta-cell function and activation is linked to increased as well as decreased phosphorylation of the enzyme PMID: 21152070
  4. PDE3 inhibition reverses leptin-induced stimulation of proopiomelanocortin and neurotensin gene expression; the PDE3B pathway plays an important role in mediating leptin action in the hypothalamus PMID: 20471454
  5. plays an important role in the stimulatory action of cAMP on pancreatic beta-cell exocytosis and release of insulin PMID: 12169692
  6. systemic administration of forskolin increased PDE3A, PDE3B, and PDE4D mRNA levels in aortic VSMC and femoral artery VSMC, 2) local delivery of dbcAMP increased PDE3A, PDE3B, and PDE4D3 protein levels in femoral artery VSMC. PMID: 12181425
  7. data suggest that PDE3B, but not PDE4, contributes to the regulation of insulin-induced glucose uptake, GLUT-4 translocation, and lipogenesis presumably by regulation of a cAMP/Epac signalling mechanisms PMID: 15961276
  8. PDE3B, a key enzyme in insulin signalling, appears to be associated with caveolae in adipocytes and this localization seems to be functionally important. PMID: 16503395
  9. insulin and beta-agonists act directly at adipocytes in opposing fashions to regulate production of adiponectin & leptin; PI3K-PDE3B-cAMP pathway mediates effects of insulin to restore beta-agonist/cAMP-suppressed secretion of these adipokines. PMID: 17286556
  10. Increased expression of PDE3B along with decreased cAMP in the exdotoxemic kidneys were associated with reduced renal function, alteration of renal tubular cell morphology, and increased iNOS expression. PMID: 19486524

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Cyclic nucleotide phosphodiesterase family, PDE3 subfamily
Tissue Specificity
Abundant in adipose tissues.
Database Links
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