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Recombinant Rhizobium sullae Copper-containing nitrite reductase (nirK)

Recombinant Rhizobium sullae Copper-containing nitrite reductase (nirK)

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Code	CSB-EP726612RBAW
Abbreviation	Recombinant Rhizobium sullae nirK protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

nirK

Uniprot No.

Q60214

Research Area

others

Alternative Names

Cu-NIR

Species

Rhizobium sullae (Rhizobium hedysari)

Source

E.coli

Expression Region

36-377aa

Target Protein Sequence

TPVRKLSATEIAALPRRKLDLVKPPFVHVHTQKAEGGPKVVEVTLTIEEKKLVIDGKGTEVNAMTFDGSVPGPLIVVHQDDYVEVTLVNPETNTLQHNIDFHSATGALGGGALTVVNPGESAVLRFKATKAGVFVYHCAPPGMVPWHVTSGMNGAIMVLPREGLTDGHGKELVYDKVYYLGEQDFYIPRDEKGEFKKYDSPGEAYEDTVAVMRTLTPTHIVFNGAVGALTGENALTAAVGERVLIVHSQANRDTRPHLIGGHGEYVWRTGKFVNVPDRDQETWFIPGPTRGAAYYTFEQPGIYAYVNHNLIEAFELGAAAHFKVTGDWNDDLMTTVRSPSGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

40.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Rhizobium sullae (Rhizobium hedysari) nirK protein is made by following sequential steps: inserting a DNA fragment encoding the Rhizobium sullae (Rhizobium hedysari) nirK protein (36-377aa) into a plasmid vector, transferring this vector into e.coli cells, selecting the positive cells, culturing positive cells, inducing the protein expression, and collecting the nirK protein. The protein features a N-terminal 6xHis tag fusion. The recombinant nirK protein is subjected to affinity purification and SDS-PAGE analysis. Its purity is over 85%.