Recombinant Saccharomyces cerevisiae Double-strand break repair protein MRE11(MRE11) ,partial

Code CSB-YP339299SVG
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Source Yeast
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Code CSB-EP339299SVG
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Source E.coli
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Code CSB-EP339299SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339299SVG
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Source Baculovirus
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Code CSB-MP339299SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MRE11
Uniprot No. P32829
Alternative Names MRE11; YMR224C; YM9959.06C; Double-strand break repair protein MRE11
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing.
Gene References into Functions
  1. error-prone BIRs are increased in homeologous recombination in wild-type and in mre11A470T cells. PMID: 29705126
  2. Resection of DNA breaks is impaired when either Sae2 activity is blocked, suggesting roles for both Mre11 and Sae2 nuclease activities in promoting the processing of DNA ends in vivo. PMID: 28970327
  3. A major DSB sensor, the Mre11-Rad50-Xrs2 (MRX) complex, is regulated by cell cycle-dependent phosphorylation specifically in mitosis. PMID: 24553123
  4. propose that Mre11 nuclease- and Sae2-dependent DNA end processing, which initiates DSB resection prevents Ku from engaging DSBs, thus promoting Exo1-dependent resection PMID: 21876003
  5. results identify a function for MRE11 in the response to topoisomerase poisons that is distinct from its functions in DSB repair or meiotic DNA processing PMID: 21060845
  6. Mre11 mutation promotes telomere recombination and an efficient bypass of senescence. PMID: 20421597
  7. Mre11 nuclease activity is essential for resection in the absence of extensive double-strand break end resection enzymes. PMID: 20834227
  8. Mre11 mutations are associated with increased rates of spontaneous loss of heterozygosity leading to genome instability. PMID: 20410660
  9. The nuclease function of Mre11 is required for RMX-mediated recombinational repair and telomere stabilization in mitotic cells. PMID: 15126391
  10. Mre11 nuclease is not involved in the extensive 5' to 3' resection of double-strand breaks PMID: 15485933
  11. Rad50 hook is critical for Mre11 complex-dependent DNA repair, telomere maintenance and meiotic double-strand break formation PMID: 15852023
  12. Srs2 and Sgs1 associate with Mre11 in different subcomplexes following checkpoint abd CDK1-mediated Srs2 phosphorylation. PMID: 15964827
  13. These data suggest that Mre 11p can act as a positive and negative regulator of TRD in context of the MRX complex that is essential for TRD. PMID: 15990364
  14. S.cerevisiae Mre11 protein possesses high binding affinity for G4 DNA and cleaves G-rich sequences. PMID: 16116037
  15. Mutations in Mre11 phosphoesterase motif I impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex stability in addition to nuclease activity. PMID: 16143598
  16. Mre11/Rad50 complexes from three organisms catalyze the reversible adenylate kinase reaction in vitro. PMID: 17349953
  17. Mre11 is involved in the regulation of a specific class of genes during spore development through its C-terminus domain. PMID: 17396017
  18. Mre11 nuclease and C-terminal tail-mediated DDR functions are required for initiating yeast telomere healing. PMID: 18335232

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Subcellular Location Nucleus
Protein Families MRE11/RAD32 family
Database Links

KEGG: sce:YMR224C

STRING: 4932.YMR224C

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