Recombinant Saccharomyces cerevisiae Origin recognition complex subunit 6 (ORC6)

Code CSB-YP017236SVG
MSDS
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Source Yeast
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Code CSB-EP017236SVG
MSDS
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Source E.coli
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Code CSB-EP017236SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017236SVG
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017236SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ORC6
Uniprot No.
Alternative Names
ORC6; AAP1; YHR118C; Origin recognition complex subunit 6; ACS-associated protein 1; Origin recognition complex 50 kDa subunit
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-435
Target Protein Sequence
MSMQQVQHCV AEVLRLDPQE KPDWSSGYLK KLTNATSILY NTSLNKVMLK QDEEVARCHI CAYIASQKMN EKHMPDLCYY IDSIPLEPKK AKHLMNLFRQ SLSNSSPMKQ FAWTPSPKKN KRSPVKNGGR FTSSDPKELR NQLFGTPTKV RKSQNNDSFV IPELPPMQTN ESPSITRRKL AFEEDEDEDE EEPGNDGLSL KSHSNKSITG TRNVDSDEYE NHESDPTSEE EPLGVQESRS GRTKQNKAVG KPQSELKTAK ALRKRGRIPN SLLVKKYCKM TTEEIIRLCN DFELPREVAY KIVDEYNINA SRLVCPWQLV CGLVLNCTFI VFNERRRKDP RIDHFIVSKM CSLMLTSKVD DVIECVKLVK ELIIGEKWFR DLQIRYDDFD GIRYDEIIFR KLGSMLQTTN ILVTDDQYNI WKKRIEMDLA LTEPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.
Gene References into Functions
  1. The Orc6 RXL/cyclin-binding motif plays a positive role in the initial recruitment of Cdt1/Mcm2-7 to the origin, suggesting that this motif is critical for the switch between active and inhibited ORC function at the G1-to-S-phase transition. PMID: 21289063
  2. Orc6 was required for entry into S phase after pre-replicative complexes formation PMID: 17053779
  3. Orc6 is required for dynamic recruitment of Cdt1 during repeated loading of DNA helicases. PMID: 18006685
Subcellular Location
Nucleus.
Protein Families
ORC6 family
Database Links

KEGG: sce:YHR118C

STRING: 4932.YHR118C

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