Recombinant Saccharomyces cerevisiae RNA polymerase II transcriptional coactivator SUB1 (SUB1)

Code CSB-YP345344SVG
MSDS
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Source Yeast
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Code CSB-EP345344SVG
MSDS
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Source E.coli
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Code CSB-EP345344SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345344SVG
MSDS
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Source Baculovirus
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Code CSB-MP345344SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SUB1
Uniprot No.
Alternative Names
SUB1; TSP1; YMR039C; YM9532.04C; RNA polymerase II transcriptional coactivator SUB1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-292
Target Protein Sequence
MSYYNRYRNK RRSDNGGGNL SNSNNNNGGM PSGLSASDAI FDLGKNKRVT VRQFRNINLI DIREYYLDSS TGEMKPGKKG ISLTEDLYDE LLKHRLNIDE ALRRLGSKRP KTKMVRLLSD DEYEDDNNND STNNDKDKNG KDKNSPKKRR EDKSKASNES HDLEPRSKKK KPAPPTLLPH EENIQNAERE ANATLIIPGQ AGRKQQEERK QKEKEEAEEA KAKAVAEQEK EAKAKEKIAE PEPEPVPTLQ AKKEDIVSNI NESKDANSSD EEFAQSLEAE MNKAEDDISE EE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in the release of TFIIB from the transcription complex during transcription initiation. Binds to TFIIB and specifically inhibits the formation of the TBP-TFIIB-promoter complexes.
Gene References into Functions
  1. Evolutionarily conserved role for Sub1 and PC4 in the maintenance of yeast and human genome stability has been summarized. (Review) PMID: 28567479
  2. Yeast Sub1 and human PC4 are G-quadruplex binding proteins that suppress genome instability at co-transcriptionally formed G4 DNA. PMID: 28369605
  3. demonstrate that purified Sub1 protein can protect DNA from oxidative damage in vitro, using the metal ion catalyzed oxidation assay PMID: 26708217
  4. This hypothesis was reinforced by the finding that inactivation of Sub1 transcription initiation factor, which is found predominantly in the regions of transcription initiation, leads to further increase in mutagenesis in the beginning of the genes. PMID: 25941824
  5. results suggest that SUB1 acts as a negative regulator of sporulation. PMID: 26147804
  6. Sub1, and its human homolog PC4, bind preferentially to G-quadruplexes PMID: 25813861
  7. Differential requirement for SUB1 in chromosomal and plasmid double-strand DNA break repair. PMID: 23554872
  8. Propose that Sub1 and RPA interact with the nontemplate strand of RNA polymerase II complexes during initiation and elongation, respectively. PMID: 22055186
  9. multiple roles for Sub1 in the regulation of RNA polymerase II C-terminal domain phosphorylation throughout the transcription cycle PMID: 20823273
  10. Sub1 influences and/or is sensitive to the phosphorylation status of elongating RNAP II. PMID: 15692559
  11. the transcriptional co-activator Sub1 also has a role in transcriptional repression during transcription initiation in vivo. PMID: 18823333
  12. during osmotic shock, Sub1 facilitates osmoresponse gene transcription by enhancing preinitiation complex formation PMID: 19204085
  13. Data showed that Sub1 was required for optimal Pol III gene transcription in exponentially growing cells. PMID: 19706510

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Subcellular Location
Nucleus.
Protein Families
Transcriptional coactivator PC4 family
Database Links

KEGG: sce:YMR039C

STRING: 4932.YMR039C

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