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CUSABIO expressed and purified the Recombinant Schistosoma mansoni Major egg antigen p40 from E. coli as an N-terminal 6xHis fusion protein. This full-length protein (1-354aa) has a high purity greater than 90%. The protein of the observed size (43-45 kDa) was identified by SDS-PAGE analysis. This recombinant major egg antigen is in-stock and thus allows faster delivery to your lab bench. In addition to being an immunogen for antibody synthesis, the protein may be used in the studies of schistosomiasis.
The Schistosoma mansoni major egg antigen p40 (Smp40) is highly immunogenic in humans. It plays a significant role in impairing the CD4+ Th cell-mediated immune responses such as the granulomatous response to tissue deposited eggs and subsequent fibrosis. This process may prevent or alleviate clinical symptoms of schistosomiasis. Furthermore, Smp40 is a promising candidate for developing an anti-pathology schistosomal vaccine.
|Purity||Greater than 90% as determined by SDS-PAGE.|
Major egg antigen; p40
|Species||Schistosoma mansoni (Blood fluke)|
|Target Protein Sequence||MSGGKQHNAVSIPVNREQRSFEKQRRDLLTGLEHGGGAHRGNSIAPYTEDWPSTVDNWIDSSWKRWDDDMRRLRRGMFALLPLDTFSIGILENPFALMHQMDRQIQDIRERMGSLDVPSTGSVNDFLKDAYEVGEDGKVHFKVRFDAQGFAPQDINVTSSENRVTVHAKKETTTDGRKCSREFCRMVQLPKSIDDSQLKCRMTDDGVLMLEAPVKVDQNQSLTLNESGQVAVRPKSDNQIKAVPASQALVAKGVHGLSYVDDGSGGKRLHVEVAVDPVYKPEDLFVNVDSNRVVVSGRHHKQKSDQHGRSSSFAEFSQSYAIPETVDPLSVSAQVVGNTLVLEAPLEKQHAITH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Protein Length||Full Length|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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