Recombinant Staphylococcus aureus Chemotaxis inhibitory protein (chp)

In Stock
Code CSB-YP746696SKV
MSDS
Size $436
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP746696SKV could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain MRSA252) chp.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP746696SKV could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain MRSA252) chp.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
chp
Uniprot No.
Research Area
others
Alternative Names
chp; SAR2036Chemotaxis inhibitory protein; CHIPS
Species
Staphylococcus aureus (strain MRSA252)
Source
Yeast
Expression Region
29-149aa
Target Protein Sequence
FTFEPFPTNEEIESNKKMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTVYLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
16.1kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Investigate host-pathogen interactions with our Recombinant Staphylococcus aureus chp, a vital player in bacterial evasion of host immune responses. This product, known as the Chemotaxis inhibitory protein (CHIPS), inhibits neutrophil chemotaxis, a key defense mechanism, thereby giving the bacterium an edge during infection. Derived from the notorious methicillin-resistant Staphylococcus aureus strain MRSA252, this protein adds significant value to your research in microbial pathogenesis.

This mature form of the protein consists of amino acids 29-149, replicating the functionally active form of the protein present in the bacterium. The expression is conducted in a yeast system, ensuring proper folding and post-translational modifications. Equipped with an N-terminal 6xHis-tag, this protein facilitates straightforward purification and application in various assays. The Recombinant Staphylococcus aureus chp passes stringent purity checks, with its purity greater than 90% as determined by SDS-PAGE. You can choose from a liquid or lyophilized powder form, as per your research requirements. Expand the horizons of your immunological research with the application of this vital bacterial protein.

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Target Background

Function
Involved in countering the first line of host defense mechanisms. Specifically inhibits the response of human neutrophils and monocytes to complement anaphylatoxin C5a and formylated peptides, like N-formyl-methionyl-leucyl-phenylalanine (fMLP). Acts by binding directly to the C5a receptor (C5aR) and formylated peptide receptor (FPR), thereby blocking the C5a- and fMLP-induced calcium responses. Prevents phagocytosis of the bacterium.
Subcellular Location
Secreted.
Protein Families
CHIPS/FLIPr family
Database Links

KEGG: sar:SAR2036

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