Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

eta

Uniprot No.

P09331

Research Area

Others

Alternative Names

etaExfoliative toxin A; EC 3.4.21.-; Epidermolytic toxin A

Species

Staphylococcus aureus

Source

E.coli

Expression Region

39-280aa

Target Protein Sequence

EVSAEEIKKHEEKWNKYYGVNAFNLPKELFSKVDEKDRQKYPYNTIGNVFVKGQTSATGVLIGKNTVLTNRHIAKFANGDPSKVSFRPSINTDDNGNTETPYGEYEVKEILQEPFGAGVDLALIRLKPDQNGVSLGDKISPAKIGTSNDLKDGDKLELIGYPFDHKVNQMHRSEIELTTLSRGLRYYGFTVPGNSGSGIFNSNGELVGIHSSKVSHLDREHQINYGVGIGNYVKRIINEKNE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.9kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Deepen your understanding of bacterial pathogenicity with the Recombinant Staphylococcus aureus eta. The product features Exfoliative toxin A (Epidermolytic toxin A), a vital virulence factor produced by Staphylococcus aureus. This toxin is a valuable research tool in the investigation of Staphylococcal infections, especially those characterized by epidermal blistering and desquamation.

This product is a full-length version of the mature protein, comprising amino acids 39-280. The expression of this protein is carried out in E.coli, and it is tagged at the N-terminus with a 6xHis-tag, which simplifies purification and identification steps. The Recombinant Staphylococcus aureus eta boasts a purity level of over 90%, confirmed by SDS-PAGE. It is offered in two forms, liquid and lyophilized powder, to accommodate diverse experimental designs. This exfoliative toxin is an excellent resource for any lab studying Staphylococcal infections and their associated disease mechanisms.

Target Background

Function

Has serine protease-like properties and binds to the skin protein profilaggrin. Cleaves substrates after acidic residues. Exfoliative toxins cause impetigous diseases commonly referred as staphylococcal scalded skin syndrome (SSSS).

Protein Families

Peptidase S1B family

Code	CSB-EP357644FKZ
Abbreviation	Recombinant Staphylococcus aureus eta protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP357644FKZ could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus eta. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP357644FKZ could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus eta.
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Recombinant Staphylococcus aureus Exfoliative toxin A (eta)

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