Recombinant Staphylococcus aureus Iron-regulated surface determinant protein B (isdB), partial

The expression region of this recombinant Staphylococcus aureus isdB covers amino acids 41-269. The expected molecular weight for the isdB protein is calculated to be 41.6 kDa. The isdB protein was expressed in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of isdB, which enables a simple process of detecting and purifying the isdB recombinant protein in the following steps.

The iron-regulated surface determinant protein B (IsdB) in Staphylococcus aureus is a crucial virulence factor involved in iron acquisition, an essential process for bacterial survival and pathogenesis. IsdB is part of the ISD system, a complex iron-scavenging machinery that enables the bacterium to acquire heme from host hemoglobin. IsdB plays a key role in binding heme and extracting iron from it, facilitating the utilization of host hemoglobin as a nutrient source. The IsdB-mediated iron acquisition is crucial for the pathogen's ability to thrive in the host environment, as iron is a limiting nutrient in the human body. Understanding the mechanisms behind IsdB and the ISD system is valuable for developing strategies to interfere with Staphylococcus aureus infection and for the design of potential therapeutic interventions. Additionally, IsdB has been explored as a potential vaccine candidate due to its role in iron acquisition and its involvement in the host-pathogen interaction.