Recombinant Staphylococcus aureus Iron-regulated surface determinant protein B(isdB),partial

Code CSB-EP313758SUM
Product Type Recombinant Protein
Size US$2466
Uniprot No. P0C7J5
Relevance Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP313758SUM could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain USA300 / TCH1516) isdB.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP313758SUM could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain USA300 / TCH1516) isdB.
Storage Buffer Tris-based buffer,50% glycerol
Alias Fur-regulated protein B Staphylococcal iron-regulated protein H Staphylococcus aureus surface protein J
Species Staphylococcus aureus (strain USA300 / TCH1516)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence AAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKT
Research Area Microbiology
Source E.coli
Gene Names isdB
Protein Names Recommended name: Iron-regulated surface determinant protein B Alternative name(s): Fur-regulated protein B Staphylococcal iron-regulated protein H Staphylococcus aureus surface protein J
Expression Region 41-269aa
Tag Info N-terminal 6xHis-SUMO-tagged
Mol. Weight 41.6kDa
Protein Description Partial
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
Subcellular Location Secreted, cell wall, Peptidoglycan-anchor
Protein Families IsdB family

Q&A and Customer Reviews

Q&A
Q:

Regarding your protein CSB-EP313758SUM, could you please provide information for all 4 expression hosts?
Also, I am wondering if it would be possible to biotinylate this protein – do you offer this modification?

A:
Thanks for your inquiry. The product details are confirmed as below.
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein B(isdB),partial
CSB-YP313758SUM >> Yeast
CSB-EP313758SUM >> E.coli
CSB-BP313758SUM >> Baculovirus
CSB-MP313758SUM >> Mammalian cell
Expression Region:41-269aa; Partial.
Tag information:EP: N-terminal 6xHis-SUMO-tagged; YP, BP, MP: Tag type will be determined during the manufacturing process.
Sequence:

AAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKT


We can provide the biotinylated service. It's labeled in vitro by random. It's labeled at the lysine free amino terminus (primaquine) not only at the N-terminus,
but the amino acids containing the lysine residue can all be labeled.
Lead time will be extended 2-3 working days.
Customer Reviews

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