Recombinant Synechococcus sp. Deoxyribodipyrimidine photo-lyase (phr)

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Code CSB-EP361494FPZ
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361494FPZ could indicate that this peptide derived from E.coli-expressed Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) phr.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361494FPZ could indicate that this peptide derived from E.coli-expressed Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) phr.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
phr
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
phr; phrA; syc1392_c; Deoxyribodipyrimidine photo-lyase; EC 4.1.99.3; DNA photolyase; Photoreactivating enzyme
Species
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Source
E.coli
Expression Region
2-484aa
Target Protein Sequence
AAPILFWHRRDLRLSDNIGLAAARAQSAQLIGLFCLDPQILQSADMAPARVAYLQGCLQELQQRYQQAGSRLLLLQGDPQHLIPQLAQQLQAEAVYWNQDIEPYGRDRDGQVAAALKTAGIRAVQLWDQLLHSPDQILSGSGNPYSVYGPFWKNWQAQPKPTPVATPTELVDLSPEQLTAIAPLLLSELPTLKQLGFDWDGGFPVEPGETAAIARLQEFCDRAIADYDPQRNFPAEAGTSGLSPALKFGAIGIRQAWRAASAAHALSRSDEARNSIRVWQQELAWREFYQHALYHFPSLADGPYRSLWQQFPWENREALFTAWTQAQTGYPIVDAAMRQLTETGWMHNRCWMIVASFLTKDLIIDWRRGEQFFMQHLVDGDLAANNGGWQWSASSGMDPKPLRIFNPASQAKKFDATATYIKRWLPELRHVHPKDLISGEITPIGRRGYPAPIVNHNLRQKQFKALYNQLKAAIAEPEAEPDS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
74.3kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Amino acids 2-484 form the expressed segment for recombinant Synechococcus sp. phr. The calculated molecular weight for this phr protein is 74.3 kDa. This protein is generated in a e.coli-based system. The N-terminal 10xHis-SUMO tag and C-terminal Myc tag was smoothly integrated into the coding gene of phr, which enables a simple process of detecting and purifying the phr recombinant protein in the following steps.

The Synechococcus sp. deoxyribodipyrimidine photo-lyase (Phr) is an enzyme that plays a critical role in the repair of DNA damage caused by ultraviolet (UV) radiation. Upon absorption of light in the visible or near-UV range, Phr undergoes a conformational change and becomes activated. The activated Phr can then bind to damaged DNA containing CPDs or 6-4 photoproducts. Through a process known as photoreactivation, the Phr enzyme utilizes energy from light to break the covalent bonds within the DNA lesions, restoring the original structure of the DNA strand. The function of Phr is particularly important for organisms like Synechococcus sp. that are exposed to natural sunlight, as it provides a mechanism to repair UV-induced DNA damage and maintain genomic integrity. Studying photo-lyases, including Synechococcus sp. Phr contributes to the understanding of DNA repair mechanisms and has implications for various biotechnological applications, such as the development of photoreactivation-based technologies for DNA repair in diverse organisms.

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Target Background

Function
Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
Protein Families
DNA photolyase class-1 family
Database Links
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