Recombinant Toxoplasma gondii Dense granule protein 2 (GRA2)

Amino acids 24-185 constitute the expression domain of recombinant Toxoplasma gondii GRA2. The expected molecular weight for the GRA2 protein is calculated to be 25.0 kDa. This GRA2 protein is produced using e.coli expression system. The GRA2 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant GRA2 protein during the following stages.

Toxoplasma gondii dense granule protein 2 (GRA2) is a crucial component of the parasitic protozoan T. gondii's dense granules, specialized secretory organelles involved in host cell invasion and establishment of the parasitophorous vacuole. GRA2 is secreted into the vacuole formed during invasion and contributes to the modification of this parasitophorous vacuole. It plays a role in the early events of host-pathogen interaction, possibly impacting host cell processes. GRA2 is recognized by the host immune system, eliciting an immune response during infection. Studies on GRA2 contribute to understanding T. gondii's pathogenesis and host-parasite interactions, providing insights into potential targets for therapeutic interventions against toxoplasmosis, a disease caused by T. gondii.