Purity
Greater than 90% as determined by SDS-PAGE.
Species
Toxoplasma gondii (strain ATCC 50611 / Me49)
Expression Region
1-674aa
Target Protein Sequence
MADSPAVGIDLGTTYSCVGVWKNDAVEIIANDQGNRTTPSYVAFTDTERLVGDAAKNQVARNPENTIFDAKRLIGRKFDDPSVQSDMKHWPFKVIAGPGDKPLIEVTYQGEKKTFHPEEVSAMVLGKMKEIAEAYLGKEVKEAVITVPAYFNDSQRQATKDAGTIAGLSVLRIINEPTAAAIAYGLDKKGCGEMNVLIFDMGGGTFDVSLLTIEDGIFEVKATAGDTHLGGEDFDNRLVDFCVQDFKRKNRGKDISTNSRALRRLRTQCERTKRTLSSSTQATIEIDSLFEGIDYSVSISRARFEELCMDYFRNSLLPVEKVLKDSGIDKRSVSEVVLVGGSTRIPKIQQLITDFFNGKEPCRSINPDEAVAYGAAVQAAILKGVTSSQVQDLLLLDVAPLSLGLETAGGVMTKLIERNTTIPTKKSQTFTTYADNQPGVLIQVYEGERAMTKDNNLLGKFHLDGIPPAPRGVPQIEVTFDIDANGIMNVTAQDKSTGKSNQITITNDKGRLSASEIDRMVQEAEKYKAEDEQNKHRVEAKNGLENYCYHMRQTLDDEKLKDKISSEDRDTANKAIQEALDWLDKNQLAEKEEFEAKQKEVESVCTPIITKLYQAGAAAGGMPGGMGGMPGGMGGMPGGMGGMPGGMGGMPGGMGGMPGAGMGGSGGPTVEEVD
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Achieving the recombinant Toxoplasma gondii (strain ATCC 50611 / Me49) HSP70 protein production entails controlling the expression of the desired gene in e.coli cells. The sequence (1-674aa) of foreign DNA is integrated into an expression vector and then transferred to e.coli cells. Selected positive cells are cultured to induce the expression of the desired protein. The protein carries a N-terminal 10xHis tag. The recombinant Toxoplasma gondii (strain ATCC 50611 / Me49) HSP70 protein is purified through affinity purification, exhibiting a purity greater than 90%, as determined by SDS-PAGE.