Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

troA

Uniprot No.

P96116

Research Area

Signal Transduction

Alternative Names

troA; troMP1; TP_0163; Periplasmic zinc-binding protein TroA; Tromp-1

Species

Treponema pallidum (strain Nichols)

Source

E.coli

Expression Region

23-308aa

Target Protein Sequence

FGSKDAAADGKPLVVTTIGMIADAVKNIAQGDVHLKGLMGPGVDPHLYTATAGDVEWLGNADLILYNGLHLETKMGEVFSKLRGSRLVVAVSETIPVSQRLSLEEAEFDPHVWFDVKLWSYSVKAVYESLCKLLPGKTREFTQRYQAYQQQLDKLDAYVRRKAQSLPAERRVLVTAHDAFGYFSRAYGFEVKGLQGVSTASEASAHDMQELAAFIAQRKLPAIFIESSIPHKNVEALRDAVQARGHVVQIGGELFSDAMGDAGTSEGTYVGMVTHNIDTIVAALAR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

38.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Treponema pallidum (strain Nichols) troA covers amino acids 23-308. The theoretical molecular weight of the troA protein is 38.2 kDa. This protein is generated in a e.coli-based system. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of troA, which enables a simple process of detecting and purifying the troA recombinant protein in the following steps.

The Treponema pallidum periplasmic zinc-binding protein TroA is a crucial component in the transport and utilization of zinc in the bacterium. As a periplasmic binding protein, TroA plays a role in binding and transporting zinc ions across the bacterial inner membrane. Zinc is an essential cofactor for various cellular processes, and its uptake is tightly regulated in bacteria. TroA is involved in the scavenging of environmental zinc, contributing to the bacterial cell's ability to adapt to varying zinc concentrations. Understanding the function of TroA in Treponema pallidum is crucial for unraveling the mechanisms of zinc homeostasis and the overall survival strategies employed by this pathogenic bacterium.

Target Background

Function

Part of an ATP-driven transport system TroABCD for zinc. Substrate-binding protein involved in the transport of zinc across the cytoplasmic membrane.

Subcellular Location

Periplasm.

Protein Families

Bacterial solute-binding protein 9 family

Database Links

KEGG: tpa:TP_0163

STRING: 243276.TP0163

Code	CSB-EP309098TPR
Abbreviation	Recombinant Treponema pallidum troA protein
MSDS	MSDS Datasheet
Size	US$388
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP309098TPR could indicate that this peptide derived from E.coli-expressed Treponema pallidum (strain Nichols) troA. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP309098TPR could indicate that this peptide derived from E.coli-expressed Treponema pallidum (strain Nichols) troA.
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Recombinant Treponema pallidum Periplasmic zinc-binding protein troA (troA)

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