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Recombinant Treponema phagedenis Treponemal membrane protein B (tmpB)

Recombinant Treponema phagedenis Treponemal membrane protein B (tmpB)

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Code	CSB-EP333469THS
Abbreviation	Recombinant Treponema phagedenis tmpB protein
MSDS	MSDS Datasheet
Size	US$388
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP333469THS could indicate that this peptide derived from E.coli-expressed Treponema phagedenis tmpB. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP333469THS could indicate that this peptide derived from E.coli-expressed Treponema phagedenis tmpB.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

tmpB

Uniprot No.

P29720

Research Area

Others

Alternative Names

tmpB; Treponemal membrane protein B; Antigen TmpB

Species

Treponema phagedenis

Source

E.coli

Expression Region

22-384aa

Target Protein Sequence

ISYDDNEYSRKSRAYTQLAEKAYDAGEYDTAIEYSQLAENFAQESAEYIKRMMARNEAEDAMNKARTRYAWAKQQKADKNYPTEYLIAGEAIKAGGIAFDNKNYDVAVTCAEKALESLKTVEPEDKVIAKAAADKAAAEKAAKEKAAREKSAKDKAAKEKAAKEKAAKDKAAKEKAAKEKAAKDKAAKEKAAKEKAAREMAAKEKAAKDKAAKEEAARKAAEEAAARKAAEEAAARKAAEEEAARIAAEEEAARKAAEEEAARKAAEEALYNEKGEKVLPSEYKVLTWKLDRECFWNIAKNPAVYNDPFMWRKLYEANKDKIPESNNPDWVEAETILVIPSIRGERREGLYDPDVKYQALPKR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The construction of a plasmid coding for the Treponema phagedenis tmpB protein (22-384aa) is the initial step for the preparation of the recombiant Treponema phagedenis tmpB protein. The next is to transform the constructed plasmid into e.coli cells. e.coli cells containing the plasmid are screened and then cultured under conditions that promote the expression of the gene of interest. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. After that, affinity purification is used to isolate and purify the recombinant tmpB protein from the cell lysate. Finally, the resulting recombinant tmpB protein undergoes SDS-PAGE analysis, demonstrating a purity greater than 85%.