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Recombinant Vibrio cholerae serotype O1 Zona occludens toxin (zot)

Recombinant Vibrio cholerae serotype O1 Zona occludens toxin (zot)

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Code	CSB-EP334425VEX
MSDS	MSDS
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

zot

Uniprot No.

P38442

Alternative Names

zot; VC_1458Zona occludens toxin; Zonular occludens toxin; Zot

Species

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Source

E.coli

Expression Region

1-399aa

Target Protein Sequence

MSIFIHHGAPGSYKTSGALWLRLLPAIKSGRHIITNVRGLNLERMAKYLKMDVSDISIEFIDTDHPDGRLTMARFWHWARKDAFLFIDECGRIWPPRLTVTNLKALDTPPDLVAEDRPESFEVAFDMHRHHGWDICLTTPNIAKVHNMIREAAEIGYRHFNRATVGLGAKFTLTTHDAANSGQMDSHALTRQVKKIPSPIFKMYASTTTGKARDTMAGTALWKDRKILFLFGMVFLMFSYSFYGLHDNPIFTGGNDATIESEQSEPQSKATVGNAVGSKAVAPASFGFCIGRLCVQDGFVTVGDERYRLVDNLDIPYRGLWATGHHIYKDTLTVFFETESGSVPTELFASSYRYKVLPLPDFNHFVVFDTFAAQALWVEVKRGLPIKTENDKKGLNSIF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

48.4kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I have the following question for Recombinant Vibrio cholerae serotype O1 Zona occludens toxin (cat#: CSB-EP334425VEX; Lot#: 03663):
Is this toxin thermolabel? Because I want to know if I can clean our lab equipment that we will be using for our experiment (using the toxin) by autoclave? Or what is the best way I can eliminate the toxin safely?

Very nice to hear from you. The order details are as below.
CSB-EP334425VEX Name: Recombinant Vibrio cholerae serotype O1 Zona occludens toxin(zot)
Seq Pos:1-399aa; Full length.
Sequence:

Tag Info:N-terminal 10xHis-tagged
Unit Size:0.05 mg (E-Coli)
Aseptic processing and Endotoxin removal
There is a literature reporting that the activity of this toxin is reversible, heat-labile, sensitive to protease digestion. Here is the link: https://europepmc.org/abstract/MED/2052603
It show that you could clean your lab equipment that you will be using for your experiment (using the toxin) by autoclave.