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Recombinant Viscum album Viscotoxin-A3 (THI2.1), partial

Recombinant Viscum album Viscotoxin-A3 (THI2.1), partial

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Code	CSB-EP365586VDO
Abbreviation	Recombinant Viscum album THI2.1 protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

THI2.1

Uniprot No.

P01538

Research Area

Allergen

Alternative Names

THI2.1; Viscotoxin-A3 [Cleaved into: Viscotoxin-A3; Acidic protein]

Species

Viscum album (European mistletoe)

Source

E.coli

Expression Region

27-72aa

Target Protein Sequence

KSCCPNTTGRNIYNACRLTGAPRPTCAKLSGCKIISGSTCPSDYPK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

20.8kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Viscum album THI2.1 was expressed with the amino acid range of 27-72. This THI2.1 protein is theoretically predicted to have a molecular weight of 20.8 kDa. The THI2.1 protein was expressed in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the THI2.1 encoding gene fragment was conducted, allowing for easier detection and purification of the THI2.1 protein in subsequent stages.

Viscum album Viscotoxin-A3 (THI2.1) is a toxic protein found in European mistletoe. It exhibits cytotoxic properties, disrupting cell membranes by forming pores and inducing cell death. This characteristic makes it a potential candidate for cancer research. The toxin selectively targets cancer cells, showing promise for developing anticancer drugs.

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Target Background

Function

Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.

Subcellular Location

Secreted.

Protein Families

Plant thionin (TC 1.C.44) family

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