Recombinant Viscum album Viscotoxin-A3 (THI2.1), partial

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Code CSB-EP365586VDO
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
THI2.1
Uniprot No.
Research Area
Allergen
Alternative Names
THI2.1; Viscotoxin-A3 [Cleaved into: Viscotoxin-A3; Acidic protein]
Species
Viscum album (European mistletoe)
Source
E.coli
Expression Region
27-72aa
Target Protein Sequence
KSCCPNTTGRNIYNACRLTGAPRPTCAKLSGCKIISGSTCPSDYPK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
20.8kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant Viscum album THI2.1 was expressed with the amino acid range of 27-72. This THI2.1 protein is theoretically predicted to have a molecular weight of 20.8 kDa. The THI2.1 protein was expressed in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the THI2.1 encoding gene fragment was conducted, allowing for easier detection and purification of the THI2.1 protein in subsequent stages.

Viscum album Viscotoxin-A3 (THI2.1) is a toxic protein found in European mistletoe. It exhibits cytotoxic properties, disrupting cell membranes by forming pores and inducing cell death. This characteristic makes it a potential candidate for cancer research. The toxin selectively targets cancer cells, showing promise for developing anticancer drugs.

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Target Background

Function
Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.
Subcellular Location
Secreted.
Protein Families
Plant thionin (TC 1.C.44) family
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