Recombinant Xenopus laevis Bone morphogenetic protein 4(bmp4)

Code CSB-YP002740XBE
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Source Yeast
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Code CSB-EP002740XBE
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Source E.coli
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Code CSB-EP002740XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002740XBE
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Source Baculovirus
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Code CSB-MP002740XBE
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names bmp4
Uniprot No. P30885
Alternative Names bmp4; dvr-4Bone morphogenetic protein 4; BMP-4
Species Xenopus laevis (African clawed frog)
Expression Region 288-401
Target Protein Sequence SPK QQRPRKKNKH CRRHSLYVDF SDVGWNDWIV APPGYQAFYC HGDCPFPLAD HLNSTNHAIV QTLVNSVNSS IPKACCVPTE LSAISMLYLD EYDKVVLKNY QEMVVEGCGC R
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Posterior-ventralizing factor in Xenopus mesoderm induction. Induces posteroventral mesoderm and counteracts dorsalizing signals such as activin.
Gene References into Functions
  1. Data indicate that bone morphogenetic protein (BMP) signaling is essential for erythroid differentiation, and in the absence of BMP signaling, precursor cells adopt an endothelial cell (EC) fate. PMID: 24100450
  2. limits homeobox gene expression in the organiser/non-organiser direction PMID: 14729483
  3. X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression PMID: 15602698
  4. BMP4-dependent expression of Xenopus Grainyhead-like 1 has a critical role in epidermal differentiation PMID: 15705857
  5. XBP1 interacts with BMP-4 and Xvent-2B promoters. PMID: 16278078
  6. connective tissue-specific expression of BMP-4 mRNA is up-regulated by sonic hedgehog (Shh) PMID: 17016847
  7. An intermediate level of BMP4 signaling is required to induce neural plate border fates. PMID: 18191829
  8. BMP4 inhibits Tolloid enzyme activity noncompetitively PMID: 19884260

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: xla:399322

UniGene: Xl.1141

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