Recombinant Xenopus laevis Cerberus (cer1)

Code CSB-YP005254XBE
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Source Yeast
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Code CSB-EP005254XBE
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Source E.coli
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Code CSB-EP005254XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005254XBE
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Source Baculovirus
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Code CSB-MP005254XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cer1
Uniprot No.
Alternative Names
cer1; cer; Cerberus; Cer; Xcer) [Cleaved into: Cerberus long; Cer-L); Cerberus short; Cer-S)]
Species
Xenopus laevis (African clawed frog)
Expression Region
21-270
Target Protein Sequence
KHSEGRERTK TYSLNSRGYF RKERGARRSK ILLVNTKGLD EPHIGHGDFG LVAELFDSTR THTNRKEPDM NKVKLFSTVA HGNKSARRKA YNGSRRNIFS RRSFDKRNTE VTEKPGAKMF WNNFLVKMNG APQNTSHGSK AQEIMKEACK TLPFTQNIVH ENCDRMVIQN NLCFGKCISL HVPNQQDRRN TCSHCLPSKF TLNHLTLNCT GSKNVVKVVM MVEECTCEAH KSNFHQTAQF NMDTSTTLHH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Inhibits wnt, nodal/nr-1 and bmp signaling in the embryo to promote head formation and anterior neural induction. Within the endoderm, acts as an essential mediator of nodal/nr-1-induced cardiogenesis in the overlying mesoderm.
Gene References into Functions
  1. Studies indicate that erberus prevents Nodal signaling in the anterior and right sides of the embryo. PMID: 19247954
  2. Cardiogenesis in mesoderm depends on Nodal-mediated induction of Cerberus in underlying endoderm. PMID: 17123501
Subcellular Location
Secreted.
Protein Families
DAN family
Tissue Specificity
A component of the Nieuwkoop signaling center in the blastula. Expressed transiently in a broad anterior domain of the gastrula, including the anterior endoderm of the Spemann's organizer and more laterally the cardiac primordia. Expression is excluded fr
Database Links

KEGG: xla:398060

UniGene: Xl.368

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