Recombinant Xenopus laevis Cysteine protease ATG4D (atg4d)

Code CSB-YP715027XBE
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Source Yeast
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Code CSB-EP715027XBE
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Source E.coli
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Code CSB-EP715027XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP715027XBE
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Source Baculovirus
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Code CSB-MP715027XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
atg4d
Uniprot No.
Alternative Names
atg4d; apg4d; Cysteine protease ATG4D; EC 3.4.22.-; Autophagin-4; Autophagy-related protein 4 homolog D
Species
Xenopus laevis (African clawed frog)
Expression Region
1-469
Target Protein Sequence
MNSVSPLATQ YGSPKGSQQM ENRSTQSGGH EQRKMGHQDA TLDGEADEVD KLKSKLLSAW NNVKYGWSVK MKTTFSRSAP VYLLGERYFF RLDDEIERFQ KDFVSRVWLT YRRDFPALEG TALTTDCGWG CMIRSGQMLL AQGLLLHLLS REWTWSEALY RHFVEMEPIR SSSPPSMPLS SLATGHSAGD YQPHTQCSGA PHGDQVHRNI MRWFSDHPGS PFGLHQLVTL GSIFGKKAGD WYGPSIVAHI IKKAIETSSE VPELSVYVSQ DCTVYKADIE QLFAGDVPHA ETSRGAGKAV IILVPVRLGG ETFNPVYKHC LKEFLRMPSC LGIIGGKPKH SLYFIGYQDN YLLYLDPHYC QPYIDTSKND FPLESFHCNS PRKISITRMD PSCTFAFYAK NSEDFGKLCD HLMKVLHSPR AEEKYPIFSI SEGQAQEYAE GPQSSSHPPV CRKKGPLVKR PSSDEFEFL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Subcellular Location
Cytoplasm.
Protein Families
Peptidase C54 family
Database Links

KEGG: xla:447241

UniGene: Xl.11066

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