Recombinant Xenopus laevis Nuclear receptor subfamily 6 group A member 1 (nr6a1)

Code CSB-YP016067XBE
MSDS
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Source Yeast
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Code CSB-EP016067XBE
MSDS
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Source E.coli
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Code CSB-EP016067XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016067XBE
MSDS
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Source Baculovirus
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Code CSB-MP016067XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nr6a1
Uniprot No.
Alternative Names
nr6a1; gcnf; Nuclear receptor subfamily 6 group A member 1; Germ cell nuclear factor; GCNF; xGCNF
Species
Xenopus laevis (African clawed frog)
Expression Region
1-435
Target Protein Sequence
MDTWEDDRVD QRACLICGDR ATGLHYGIIS CEGCKGFFKR SICNKRVYRC SRDKNCVMSR KQRNRCQYCR LLKCLQMGMN RKAIREDGMP GGRNKSIGPV QISDEEVERI MSGQEFEEEA NTSWSNNGDS DHSSPGNAVS ESNQPSPVST PSSSRSMELN GFSSLRDQYL GTPGPTHYQY LPHLFSFSAH PTIIPAQSRS LDPQSHTLIN QLLTAEDIEP LNTPMLIEDG YKVTQSELFA LLCRLADELL FRQITWVKKL PFFCDLSIKD YTCLLSTTWQ ELILLSSITT YSKQIFGDLT DVTSKYSPSE DELHRFSEDG MEVMERLIYL FRKFSQLKVS NEEYVCMKAI NFLNQDIQGI SSASQVEQLN KRYWYVCQDF TEYRYPHQPN RFPDLMMCLP EVRYIAGKLV NVPLEQLPLL FKAFLHSCKT IVTKE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable orphan nuclear receptor. Binds to a response element containing repeats of the motif 5'-AGGTCA-3'. Required for anterior-posterior patterning during organogenesis. Acts with chordin to play a role in patterning the midbrain-hindbrain. Isoform Em is required for integrin-mediated cell matrix interaction during neurulation and for the morphogenetic movements leading to formation of the neural tube. Also mediates the effect of retinoic acid on primary neurogenesis.
Gene References into Functions
  1. evidence pesented that illustrates the function of germ cell nuclear factor in the early development of the CNS [GCNF] PMID: 12676320
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Nuclear hormone receptor family, NR6 subfamily
Tissue Specificity
Transiently expressed in differentiating cells of all embryonic germ layers. Expressed in an anterior to posterior concentration gradient from late gastrula to midneurula stages. Shows a complicated spatio-temporal pattern of expression during neurulation
Database Links

KEGG: xla:399304

UniGene: Xl.37734

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