Recombinant Xenopus laevis Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform (ppp2r2b)

Code CSB-YP018565XBE
MSDS
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Source Yeast
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Code CSB-EP018565XBE
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Source E.coli
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Code CSB-EP018565XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018565XBE
MSDS
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Source Baculovirus
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Code CSB-MP018565XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ppp2r2b
Uniprot No.
Alternative Names
ppp2r2b; ppp2r2b-a; ppp2r2cSerine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform; PP2A subunit B isoform B55-beta; PP2A subunit B isoform PR55-beta; PP2A subunit B isoform R2-beta; PP2A subunit B isoform beta
Species
Xenopus laevis (African clawed frog)
Expression Region
1-468
Target Protein Sequence
MPCSVEDKTG LCGSRYSVMI PELQESVIFS EDSRHYNEKQ TRRQRKSSAD IISTVEFNNT GELLATGDKG GRVVIFQREQ ENKNQPHRRG EYNVYSTFQS HEPEFDYLKS LEIEEKINKI RWLPQQNAAY FLLSTNDKTV KLWKVSERDK RPEGYNLKDE EGRIRDPCTI TSLRVPVLRP MDLMVEATPR RVFSNAHTYH INSISVNSDY ETYMSADDLR INLWNLEITN RSFNIVDIKP TNMEELTEVI TAAEFHPHNC NTFVYSSSKG TIRLCDMRSS ALCDKHSKLF EEPEDPSNRS FFSEIISSIS DVKFNHSGRY IMTRDYLTVK VWDLNMENRP IETYQVHDYL RSKLCSLYEN DCIFDKFECV WNGSDSVIMT GSYNNFFRMF DRNTKRDVTL EASRENSKPR AILKPRKVCV GGKRRKDEIS VDSLDFSKKI LHTAWHPSEN IIAVAATNNL YIFQDKVN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Negatively control the initiation of oocyte maturation.
Gene References into Functions
  1. Together, these results indicate that capsaicin inhibits the patterning of the dorso-ventral and anterior-posterior body axes of embryo by repressing PP2A and thereby down-regulating the Wnt/beta-catenin signaling. PMID: 27318088
  2. study describes how Gwl activation results in PP2A inhibition; identified Arpp19 and alpha-Endosulfine that, when phosphorylated by Gwl, associate with and inhibit PP2A promoting mitotic entry PMID: 21164014
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Membrane.
Protein Families
Phosphatase 2A regulatory subunit B family
Database Links

KEGG: xla:394301

UniGene: Xl.429

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