Recombinant Xenopus tropicalis Deleted in azoospermia-like(dazl)

Code CSB-YP006514XBF
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Source Yeast
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Code CSB-EP006514XBF
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Source E.coli
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Code CSB-EP006514XBF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006514XBF
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Source Baculovirus
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Code CSB-MP006514XBF
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names dazl
Uniprot No. Q76CY5
Alternative Names dazlDeleted in azoospermia-like; DAZ-like protein; Xtdazl
Species Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region 1-286
Target Protein Sequence MSATEASAGE EAASATSQAF VLPEGKVIPN TVFVGGIDIT MDEMAIGNLF EKYGKVKDLK IITDRTGVSK GYGFVSFYDE VDVQKIVKSQ INFHGKKLKL GPAIRKMQRI CTYVQTSPVV ISPPTQFHPT WNSQNADSYI QHSPIISPVT QYVQACPYPS SPPMAIQQIP VGCQQPSYFQ VSPQWPTDQR NYMFPSPAFT FNYNCCEIDP NGGEPMPREY HIDQAVSASG ANLQKRYVDM STQTIISCLF DPAKKVRLFA SQEDYIQDDS VHQFRRSKSV IKRVSN
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function RNA-binding protein that is required for primordial germ cell (PGC) differentiation and indirectly necessary for the migration of PGCs through the endoderm. May promote meiotic cell division during spermatogenesis. Shows a preference for G- and U-rich RNAs and probably binds the 3'-UTR of target mRNAs. Stimulates the initiation of translation of mRNAs through the recruitment of poly(A)-binding proteins (PABPs) (By similarity).
Gene References into Functions
  1. Xtdazl mRNA is the first specific marker of germ plasm and presumptive primordial germ cell PMID: 14745962
Subcellular Location Cytoplasm
Protein Families RRM DAZ family
Tissue Specificity Germ-line specific; expressed in adult testis and ovary. Localized specifically to the oocyte and embryonic germ plasm and to migrating primordial germ cells (PGCs).
Database Links

KEGG: xtr:394676

STRING: 8364.ENSXETP00000053944

UniGene: Str.6165

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