Product Details

Uniprot No.

P53396

Target Names

ACLY

Alternative Names

ATP-citrate synthase (EC 2.3.3.8) (ATP-citrate (pro-S-)-lyase) (ACL) (Citrate cleavage enzyme), ACLY

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ATP citrate lyase

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3A5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ATP-citrate lyase serves as a critical metabolic enzyme that bridges cellular energy production with biosynthetic pathways by converting citrate into acetyl-CoA, the essential building block for fatty acid synthesis and protein acetylation. This enzymatic activity positions ACLY at the intersection of cancer metabolism, lipid biosynthesis, and epigenetic regulation, making it a compelling target for researchers investigating metabolic reprogramming in disease states and normal cellular physiology.

This recombinant monoclonal antibody, generated from clone 3A5, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, researchers can expect uniform performance across experiments and studies, eliminating the lot-to-lot variability that can complicate long-term projects or multi-site collaborations.

Validation across multiple detection platforms demonstrates genuine experimental flexibility. Western blot analysis confirms robust detection of ACLY at the expected 120 kDa molecular weight across diverse human cell lines including L02, K562, HeLa, Raji, and A549, providing confidence when working with hepatic, hematopoietic, cervical, or lung cancer models. Immunofluorescence staining in HepG2 cells reveals clear cytoplasmic localization patterns, while flow cytometry analysis in HeLa cells shows distinct positive population shifts compared to isotype controls, enabling quantitative single-cell studies.

The antibody's performance across these orthogonal techniques supports investigations spanning cancer biology, metabolic regulation, and signal transduction research. Whether examining ACLY expression changes in response to metabolic stress, validating knockdown efficiency, or characterizing its role in acetyl-CoA-dependent processes, this reagent provides the technical foundation for meaningful experimental insights.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA712206A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate All lanes: ACLY antibody at 1:1500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 121, 120, 92 kDa Observed band size: 120 kDa Immunofluorescence staining of HepG2 Cells with CSB-RA712206A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay histogram showing Hela cells stained with CSB-RA712206A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ACLY Recombinant Monoclonal Antibody

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