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| Code | CSB-RA712206A0HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IF | 1:20-1:200 |
| FC | 1:20-1:200 |
The ACLY recombinant monoclonal antibody is generated using both protein and DNA recombinant technology. Firstly, a synthesized peptide derived from human ACLY was used to immunize mice, from which the spleen was extracted under aseptic conditions. The total RNA was extracted from spleen cells. The obtained cDNA synthesized by RNA reverse transcription served as the template for PCR amplification of the ACLY antibody gene. The ACLY antibody gene was then introduced into a vector, which was subsequently transfected into host cells for cultivation. Following cultivation, the ACLY recombinant monoclonal antibody was isolated and purified from the supernatant of the cell culture using affinity chromatography. It underwent rigorous verification and is suitable for detecting human ACLY protein in ELISA, WB, IF, and FC experiments.
The ATP citrate lyase (ACLY) protein is an enzyme that plays a key role in cellular metabolism, particularly in fatty acid synthesis. It is responsible for catalyzing the formation of acetyl-CoA from citrate, which is generated during the citric acid cycle within the mitochondria of cells. In addition to its role in lipid metabolism, ACLY has also been implicated in the regulation of gene expression and epigenetic modifications.
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