Product Details

Uniprot No.

Target Names

Alternative Names

Activin A receptor type 1B antibody; Activin A receptor type II like kinase 4 antibody; Activin A type 1B receptor antibody; Activin A type IB receptor antibody; Activin receptor like kinase 4 antibody; Activin receptor type 1B antibody; Activin receptor type IB antibody; Activin receptor type-1B antibody; Activin receptor-like kinase 4 antibody; ACTR IB antibody; ACTR-IB antibody; ACTRIB antibody; ACV1B_HUMAN antibody; ACVR 1B antibody; Acvr1b antibody; ACVRLK 4 antibody; ACVRLK4 antibody; ALK 4 antibody; ALK-4 antibody; ALK4 antibody; Serine(threonine) protein kinase receptor R2 antibody; Serine/threonine-protein kinase receptor R2 antibody; SKR 2 antibody; SKR2 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human ACVR1B protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1D1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ACVR1B, also known as activin receptor type-1B or ALK4, serves as a critical serine/threonine kinase receptor in the TGF-β superfamily signaling pathway. This receptor plays essential roles in activin-mediated signal transduction, influencing diverse biological processes including cell proliferation, differentiation, and apoptosis. Its involvement in developmental biology and disease pathogenesis makes ACVR1B a significant target for researchers investigating signal transduction mechanisms.

This recombinant monoclonal antibody, clone 1D1, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a sequence-defined approach, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies.

Validated across multiple detection platforms, this antibody demonstrates reliable performance in Western blot, immunohistochemistry, and immunofluorescence applications. Western blot analysis confirms detection of ACVR1B at the expected 57 kDa molecular weight across a diverse panel of samples, including human cell lines such as MCF7, HEK293, U251, and COLO205, as well as mouse and rat brain tissue lysates. This cross-species reactivity spanning human, mouse, and rat samples provides flexibility for comparative studies and translational research models.

Immunohistochemical staining has been validated in paraffin-embedded human gastric cancer tissue, while immunofluorescence analysis demonstrates clear cytoplasmic localization in A549 cells. The unconjugated format allows researchers to select secondary detection systems optimized for their specific experimental requirements.

For investigators exploring TGF-β pathway dynamics, activin signaling, or receptor kinase biology, this antibody delivers the specificity and versatility needed to advance signal transduction research with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.

Gene References into Functions

Overexpression of ALK4 suppressed glioma cell proliferation, migration and invasion through the inactivation of JAK/STAT3 signaling pathway. PMID: 29278854
Results showed that ACVR1B expression is upregulated during latent infection with a miR-UL148D deletion virus. Data indicates miR-UL148D inhibits ACVR1B expression in latently infected cells to limit proinflammatory cytokine secretion. PMID: 27491954
Our study provided experimental and clinical evidence for the involvement of activin A and ALK4 in the pathophysiology of atrial fibrosis and atrial fibrillation PMID: 28639003
It was concluded that ALK4 inhibition increases myogenesis but also regulates the tight balance of protein synthesis and degradation. PMID: 27733450
in a relatively large cohort of athletes from Europe and South America we have shown that the ACVR1B rs2854464 A allele is associated with sprint/power performance in Caucasians but not in Brazilian athletes. PMID: 27253421
ALK4 is an important profibrotic signaling molecule in the post-MI CF. Haplodeficiency of ALK4 significantly improved LV function and survival rate by attenuating CF in vivo, ameliorated PMID: 28214509
ALK4 is expressed in male germ cells and Sertoli cells. PMID: 26289399
This study demonstrated positive regulation of monocyte/macrophage differentiation by lnc-MC and uncovered an elaborate regulation mechanism composed of PU.1, lnc-MC, miR-199a-5p, and ACVR1B. PMID: 26149389
Data suggest that activin A up-regulates SNAIL expression via ALK4/ACVR1B-induced SMAD signaling in trophoblast cells; elevated SNAIL contributes to up-regulation of MMP2 expression which plays key role in promoting trophoblast cell invasion. PMID: 26305619
ACVR1B functions as a positive regulator of monocyte/macrophage differentiation. ACVR1B knockdown promoted THP-1 proliferation by increasing Rb phosphorylation. Down-regulation reduced p-Smad2/3 and C/EBPalpha, inhibiting monocyte/macrophage differentiation. PMID: 25258381
The deletion of the ACVR1B gene may mediate an aggressive cancer phenotype in pancreatic cancer. PMID: 24886203
Findings define a regulatory role of miR-98 in tumor angiogenesis and invasion through repressed ALK4 and MMP11 expression. PMID: 23211491
ActRIBCA signaling can promote cell migration in prostate cancer cells via a network of signaling molecules that work together to trigger the process of EMT, and thereby aid in the aggressiveness and progression of prostate cancers. PMID: 23159635
Act A protein levels were significantly higher in NTM tissues compared to GTM tissues. PMID: 23010638
Activin A-ACVRIB/ALK4-Smad-dependent collagen production was augmented in SSc fibroblasts, suggesting the involvement of this signaling mechanism in systemic sclerosis. PMID: 21377836
We applied a two-stage fine mapping approach, and are the first to identify and partially replicate genetic variants in the ACVR1B gene that account for genetic variation in human muscle strength. PMID: 21063444
activin A signal is transduced through the activin A type 1 receptor, ALK4, and transactivates several TGF-beta target genes in a SMAD-independent manner. PMID: 20226172
data indicate that truncated Alk4 isoforms interfere with activin signaling pathways and thereby may contribute to uncontrolled cell growth PMID: 12364468
Activin signaling mediated by ActRIB-Smad2 system in the ovary may thus be essential for the regulation of follicular differentiation. PMID: 12639945
there is only a partial overlap of the binding sites on ALK4 and ALK3 for activin-A and bone morphogenetic protein-2, respectively PMID: 12665502
ALK4 binds to activin at a specific site in order to signal via type II receptors PMID: 15123686
Dpr2 binds to the TGFbeta receptors ALK5 and ALK4, and accelerates lysosomal degradation of these receptors PMID: 15459392
findings indicate a new inhibitory function of FKBP12 as an adaptor molecule for the Smad7-Smurf1 complex to regulate the duration of the activin signal through activin type I receptors PMID: 16720724
a regulation mode of miR-24 on erythropoiesis by impeding ALK4 expression. PMID: 17906079
Study indicate that the synthetic h-CFC interacts with the ALK4 receptor with a K(D) in micro M range, that the h-CFC overall topology is determined by the presence of three disulfide bridges. PMID: 19035567

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Involvement in disease

ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis.

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily

Tissue Specificity

Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.

Database Links

HGNC: 172

OMIM: 601300

KEGG: hsa:91

UniGene: Hs.438918

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Code	CSB-RA053883A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:MCF7 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), U251 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse brainr tissue lysate(30µg), Rat brain lysate(30µg) All lanes: ACVR1B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time：30s IHC image of CSB-RA053883A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA053883A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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ACVR1B Recombinant Monoclonal Antibody

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Target Background