Code | CSB-RA906343A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
B cells were obtained by immunizing an animal with a synthesized peptide derived from human ADAR, which was then fused with myeloma cells to form hybridomas. Sequencing the cDNA for ADAR antibody-producing hybridomas' variable light (VL) and variable heavy (VH) domains. This sequencing data was used for vector construction in the recombinant generation. The ADAR monoclonal antibody-containing vector was transfected into cells for culture. The ADAR recombinant monoclonal antibody was collected and purified from the cell culture supernatant using affinity chromatography. The purified antibody's specificity was tested in ELISA and IHC applications, and it was found to only detect human ADAR protein.
The ADAR protein is an RNA-editing enzyme that catalyzes the conversion of adenosine (A) to inosine (I) in double-stranded RNA (dsRNA). This editing process can alter the coding potential, splicing, and stability of RNAs. In particular, ADAR-mediated RNA editing has been shown to play important roles in regulating gene expression, innate immunity, and neuronal signaling.
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