ADH4 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA145644A0HU
Size	US$210
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Image	Immunofluorescence staining of HepG2 cell with CSB-RA145644A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA145644A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P08319

Target Names

ADH4

Alternative Names

Ac1002 antibody; ADH 4 antibody; ADH-1 antibody; ADH-2 antibody; ADH2 antibody; Adh4 antibody; ADH4_HUMAN antibody; Alcohol dehydrogenase 4 (class II) pi polypeptide antibody; Alcohol dehydrogenase 4 antibody; Alcohol dehydrogenase class II pi chain antibody; Alcohol dehydrogenase pi isozyme ADH class II antibody; Aldehyde reductase antibody; Class II alcohol dehydrogenase 4 pi subunit antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ADH4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7A5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Alcohol dehydrogenase 4 (ADH4) belongs to the class II alcohol dehydrogenase family and plays a central role in ethanol metabolism, catalyzing the oxidation of alcohols to aldehydes in the liver. Beyond its well-characterized function in alcohol processing, ADH4 also exhibits aldehyde reductase activity, positioning it as a relevant target for researchers investigating hepatic metabolism, toxicology, and signal transduction pathways. Understanding ADH4 expression and localization provides valuable insights into metabolic disorders, liver function, and individual variations in alcohol metabolism.

This recombinant monoclonal antibody, clone 7A5, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human ADH4 and has been validated for human sample reactivity, making it particularly suited for clinical and translational research applications.

Validation studies demonstrate reliable performance across multiple detection platforms. Immunofluorescence analysis of HepG2 cells, a human hepatocellular carcinoma line with preserved hepatic characteristics, reveals clear cytoplasmic staining at dilutions of 1:50 to 1:200. Flow cytometry experiments using the same cell line confirm specific detection, with distinct positive population shifts compared to isotype controls when used at 1:50 to 1:200 dilutions. The antibody is additionally compatible with ELISA applications, providing flexibility for quantitative expression studies.

Supplied in a stabilized liquid format with glycerol for convenient long-term storage, this affinity-purified antibody supports researchers exploring hepatic metabolism, alcohol-related pathologies, and broader signal transduction mechanisms involving ADH4.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Also catalyzes the reduction of benzoquinones.

Gene References into Functions

Association between HCRTR2, ADH4,CLOCK gene polymorphisms and cluster headache was not significant in the present study. PMID: 29318394
this current study provides substantial evidence that genetic polymorphisms of rs3805322 in the ADH4 gene may be associated with an increased risk of developing ESCC in two Chinese Han populations. PMID: 26039424
This study confirmed the genetic heterogeneity of cluster headache, suggesting that mutations in the ADH4 gene might be related to cluster headache in a subset of cases. PMID: 24469609
The serum expression of ADH4 protein correlates with the progression of esophageal cancer. PMID: 24051444
expression levels of ADH4 mRNA and protein have found to be markedly reduced in hepatocellular carcinoma (HCC) tissues and significantly associated with survival. PMID: 22147505
data suggest that ADH4 intronic variants play a role in alcohol dependence susceptibility in Italian populations PMID: 22044940
Our results suggest that the analysed polymorphisms ofANKK1 and ADH4 can play an important part in the pathogenesis of alcohol abuse PMID: 22232963
the high catalytic activity of human alcohol dehydrogenase 4 studied with horse liver ADH1E PMID: 21184752
This study confirms the significant relationship of ADH4 variants with AD and related phenotypes. PMID: 20626721
Data indicate that further studies of EPHX1 rs1051740, ADH4 r1042364, and NQO1 rs291766 are needed to make more definitive conclusion on the role of variation in xenobiotic metabolism in ovarian cancer. PMID: 21480392
Our data suggest that cluster headache is associated with the ADH4 gene or a linked locus. Additional studies are warranted to elucidate the role of this gene in the etiopathogenesis of the disease. PMID: 19925625
In this study we have identified a distal upstream enhancer, 4E, of ADH4. In HepG2 human hepatoma cells, 4E increased the activity of an ADH4 basal promoter by 50-fold. 4E was cell-specific. PMID: 20363298
ADH4 gene polymorphisms are associated with upper aerodigestive tract cancers. PMID: 19861527
Thirteen new single nucleotide polymorphisms PMID: 11916005
Identification and characterisation of two allelic forms of human alcohol dehydrogenase 2 PMID: 11964133
kinetic mechanisms for ADH4 functioning as retinol dehydrogenase PMID: 11997393
Results describe the expression patterns of alcohol dehydrogenases I, III, and IV in adult rat, mouse and human tissues using radioactive oligonucleotide in situ hybridization. PMID: 12631290
The mean alcohol-elimination rate among ADH2*2 carriers is significantly higher than among ADH2*1 homozygotes in a male Jewish population. PMID: 14745297
the genotypes of six ADH4 markers were associated with alcohol dependence, and all seven ADH4 markers were associated with drug dependence PMID: 16220108
Seven single nucleotide polymorphisms spanning the ADH4 gene were genotyped in 561 subjects affected with alcohol dependence and/or drug dependence. PMID: 16237392
that personality traits and substance dependence have a partially overlapping genetic basis. PMID: 17069770
Class II alcohol dehydogenase (ADH4) is considered to contribute to ethanol oxidation in the liver at high concentration. PMID: 19182438

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Subcellular Location

Cytoplasm.

Protein Families

Zinc-containing alcohol dehydrogenase family, Class-II subfamily

Database Links

HGNC: 252

OMIM: 103740

KEGG: hsa:127

STRING: 9606.ENSP00000265512

UniGene: Hs.1219

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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