Product Details

Uniprot No.

Target Names

APPL1

Alternative Names

Adapter protein containing PH domain antibody; Adaptor Protein Containing pH Domain antibody; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody; Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody; Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody; AKT2 INTERACTOR antibody; APPL 1 antibody; Appl1 antibody; DCC interacting protein 13 alpha antibody; DCC-interacting protein 13-alpha antibody; DIP 13 alpha antibody; DIP13 alpha antibody; Dip13-alpha antibody; DIP13alpha antibody; DP13A_HUMAN antibody; KIAA1428 antibody; PTB domain and leucine zipper motif 1 antibody; PTB Domain and Leucine Zipper Motif antibody; Signaling adaptor protein DIP13alpha antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human APPL1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

24F7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

APPL1 serves as a critical signaling adaptor protein that bridges multiple cellular pathways, including those involving AKT2 and the DCC receptor. This multidomain protein, containing PH, PTB, and leucine zipper motifs, plays essential roles in cell signaling, endosomal trafficking, and metabolic regulation, making it a valuable target for researchers investigating signal transduction mechanisms and cellular metabolism.

This recombinant monoclonal antibody, clone 24F7, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers can expect uniform performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Extensive validation demonstrates this antibody's versatility across multiple applications. Western blot analysis confirms reliable detection of APPL1 in diverse human cell lines, including HeLa, Jurkat, SH-SY5Y, Colo205, A431, and THP-1 lysates, with an observed band at approximately 85 kDa. The slight difference from the predicted 80 kDa molecular weight likely reflects post-translational modifications such as glycosylation, which is consistent with APPL1's known biology. Immunohistochemistry validation in paraffin-embedded human pancreatic tissue and cervical cancer specimens demonstrates suitability for tissue-based studies. Additionally, immunofluorescence staining in HeLa cells and flow cytometry analysis in A431 cells confirm utility for subcellular localization studies and quantitative single-cell analysis.

For cell biology researchers investigating adaptor protein function, endosomal signaling, or metabolic pathways, this antibody provides a reliable tool with demonstrated performance across complementary techniques, enabling comprehensive characterization of APPL1 expression and localization in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages. Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex.

Gene References into Functions

Present study demonstrated that expression of APPL1 protein and mRNA was upregulated in gastric carcinoma (GC) tissues and cell lines. The expression of APPL1 in GC was statistically associated with metastasis stage. Overexpression of APPL1 promotes invasion and metastasis of gastric cancer cells and the underlying molecular mechanism may facilitate EMT via Akt2 phosphorylation. PMID: 28902365
APPL1 positively mediated leptin signaling. PMID: 27820851
Decreased expression of APPL1 is associated with polycystic ovary syndrome. PMID: 27075719
Results indicate that persistent rab5 overactivation through beta-cleaved carboxy-terminal fragment of APP-APPL1 interactions constitutes a novel APP-dependent pathogenic pathway in Alzheimer's disease PMID: 26194181
Data show that signal transducing adaptor proteins APPL1 and APPL2 are required for TGFbeta-induced nuclear translocation of TGFbeta type I receptor (TbetaRI)-ICD and for cancer cell invasiveness of prostate and breast cancer cell lines. PMID: 26583432
Low expression of APPL1 is associated with metastasis in prostate cancer. PMID: 26473288
APPL1 endosomes represent a distinct population of Rab5-positive sorting endosomes. PMID: 26459602
article provides evidence that GG genotype and G carrier (CG+GG) genotypes of the rs4640525 polymorphism in the APPL1 gene may be suitable susceptibility biomarkers for NAFLD PMID: 26731990
Two loss-of-function mutations in APPL1, identified by means of whole-exome sequencing, found in two large families with a high prevalence of Familial Diabetes Mellitus. PMID: 26073777
A role for APPL1 in TLR3/4-dependent TBK1 and IKKepsilon activation in macrophages. PMID: 25780039
ATM is the central modulator of APPL-mediated effects on radiosensitivity and DNA repair. PMID: 24763056
APPL1 sensitizes insulin signaling by acting at a site downstream of the IR. Study uncovers a mechanism regulating insulin signaling and crosstalk between the insulin and adiponectin pathways. PMID: 24813896
APPL1 is a positive regulator of Dvl2-dependent transcriptional activity of AP-1. PMID: 25622892
It concludes that APPL1(PH) binding to BAR domain and Reptin is mutually exclusive which regulates the nucleocytoplasmic shuttling of Reptin. PMID: 23891720
The activated EGF receptor enters distinct sub-populations of SNX15- and APPL1-labelled peripheral endocytic vesicles. PMID: 23986476
C-APPL1/A-APPL2 allele combination is associated with non-alcoholic fatty liver disease occurrence, with a more severe hepatic steatosis grade and with a reduced adiponectin cytoprotective effect on liver. PMID: 23977033
TRAF6-mediated ubiquitination of APPL1 is a vital step for the hepatic actions of insulin through modulation of membrane trafficking and activity of Akt. PMID: 23909487
Rab5a and APPL1 are overexpressed in breast cancer, and are positively correlated with the HER-2 expression. PMID: 23291133
neurons with APPL1-positive granules were restricted to the CA1 area and subiculum, areas associated with hippocampal vulnerability, suggesting a possible link between the perisomatic accumulation of APPL1 and Alzheimer's disease. PMID: 23246927
APPL1 regulates basal NF-kappaB activity by modulating the stability of NIK, which affects the activation of p65. PMID: 22685329
analysis of APPL1 and APPL2 proteins and their interaction with Rab PMID: 23055524
APPL1 is a novel target in endoplasmic reticulum (ER) stress-induced insulin resistance and PKCalpha is the kinase mediating ER stress-induced phosphorylation of APPL1 at Ser(430). PMID: 22685300
results demonstrate an important new function for APPL1 in regulating cell migration and adhesion turnover through a mechanism that depends on Src and Akt PMID: 22379109
Genetic variation(s) in APPL1/2 may be associated with CAD risk in T2DM in Chinese population. PMID: 22340213
Treating C2C12 myotubes with adiponectin promoted APPL1 interaction with protein phosphatase 2A (PP2A) and protein kinase Czeta (PKCzeta), leading to the activation of PP2A and subsequent dephosphorylation and inactivation of PKCzeta. PMID: 21835890
These findings suggest that APPL1 is required for EGFR signaling by regulation of EGFR stabilities through inhibition of Rab5. PMID: 22037462
Data suggest that although annexin A2 is not an exclusive marker of APPL1/2 endosomes, it has an important function in membrane recruitment of APPL proteins, acting in parallel to Rab5. PMID: 21645192
APPL1 plays a key role in coordinating the vasodilator and vasoconstrictor effects of insulin by modulating Akt-dependent NO production and ERK1/2-mediated ET-1 secretion in the endothelium. PMID: 21926268
APPL1 abundance is significantly higher in type 2 diabetic muscle; Improvements in hyperglycaemia and hypoadiponectinaemia are associated with reduced skeletal muscle APPL1, and increased plasma adiponectin levels and muscle AMPK phosphorylation. PMID: 21562756
Results show that APPL1 is recruited to aggresomes induced by proteasomal stress, and suggest that proteasome inhibitors in clinical use affect the localization, ubiquitination and solubility of APPL1. PMID: 21320486
The adapter protein APPL1 links FSH receptor to inositol 1,4,5-trisphosphate production and is implicated in intracellular Ca(2+) mobilization. PMID: 21285318
Data indicate APPL1 functions as a scaffolding protein to facilitate adiponectin-stimulated p38 MAPK activation in myotubes. PMID: 20978232
[REVIEW] Emerging roles for AppL1. APPL1 has been shown to interact with a variety of membrane receptors. Recent subcellular localizations of APPL1 place it in dynamic and varied venues in the cell: the cell membrane, the nucleus and the early endosomes PMID: 20600589
Studies indicate that APPL1 has been recently identified as an AdipoR1 and AdipoR2 binding protein. PMID: 20875820
significant fluorescence resonance energy transfer between APPL minimal BAR domain FRET pairs PMID: 20814572
The promyogenic function of Cdo involves a coordinated activation of p38MAPK and Akt via association with scaffold proteins, JLP and Bnip-2 for p38MAPK and APPL1 for Akt. PMID: 20484574
Rab5a promoted proliferation of ovarian cancer cells, which may be associated with the APPL1-related epidermal growth factor signaling pathway. PMID: 20412119
Used mass spectrometry (MS) to identify 13 phosphorylated residues within APPL1. PMID: 20095645
identification of a pathway directly linking the small GTPase Rab5, a key regulator of endocytosis, to signal transduction and mitogenesis via APPL1 and APPL2, two Rab5 effectors PMID: 15016378
APPL1 is a potential interactor with FSHR PMID: 15070827
APPL1 interacts with adiponectin receptors in mammalian cells and the interaction is stimulated by adiponectin. PMID: 16622416
APPL1 acts as a common downstream effector of Adiponectin receptors R1 and -R2, mediating adiponectin-evoked endothelial nitric oxide production and endothelium-dependent vasodilation. PMID: 17287464
The ability of APPL1 to interact with multiple signaling molecules and phospholipids supports an important role for this adaptor in cell signaling. PMID: 17502098
The crystal structures of human APPL1 N-terminal BAR-PH domain motif, is reported. PMID: 17581628
These data suggest that APPL1 plays an important role in insulin-stimulated Glut4 translocation in muscle and adipose tissues and that its N-terminal portion may be critical for APPL1 function. PMID: 17848569
The findings suggest a role for APPL1 and APPL2 protein as dynamic scaffolds that modulate RAB5-associated signaling endosomal membranes by their ability to undergo domain-mediated oligomerization, membrane targeting and phosphoinositide binding. PMID: 18034774
Thus, binding to APPL1 helps localize OCRL at specific cellular sites, and disruption of this interaction may play a role in disease. PMID: 18307981
Adiponectin blocks interleukin-18-mediated endothelial cell death via APPL1-dependent AMP-activated protein kinase (AMPK) activation and IKK/NF-kappaB/PTEN suppression. PMID: 18632660
APPL proteins exert their stimulatory effects on beta-catenin/TCF-dependent transcription by decreasing the activity of a Reptin-containing repressive complex PMID: 19433865
Adiponectin activates AMP-activated protein kinase in muscle cells via APPL1/LKB1-dependent and phospholipase C/Ca2+/Ca2+/calmodulin-dependent protein kinase kinase-dependent pathways PMID: 19520843

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Involvement in disease

Maturity-onset diabetes of the young 14 (MODY14)

Subcellular Location

Early endosome membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endosome. Cell projection, ruffle. Cytoplasmic vesicle, phagosome.

Tissue Specificity

High levels in heart, ovary, pancreas and skeletal muscle.

Database Links

HGNC: 24035

OMIM: 604299

KEGG: hsa:26060

STRING: 9606.ENSP00000288266

UniGene: Hs.476415

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Code	CSB-RA238916A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in: Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), colo205 whole cell lysate(30µg), A431 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: Dip13 alpha antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 80 kDa Observed band size: 85 kDa Exposure time：10s IHC image of CSB-RA238916A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA238916A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA238916A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A431 cells stained with CSB-RA238916A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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APPL1 Recombinant Monoclonal Antibody

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